Soybean variety XR28L10

ABSTRACT

A novel soybean variety, designated XR28L10 is provided. Also provided are the seeds of soybean variety XR28L10, cells from soybean variety XR28L10, plants of soybean XR28L10, and plant parts of soybean variety XR28L10. Methods provided include producing a soybean plant by crossing soybean variety XR28L10 with another soybean plant, methods for introgressing a transgenic, mutant trait, and/or native trait into soybean variety XR28L10, methods for producing other soybean varieties or plant parts derived from soybean variety XR28L10. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety XR28L10 are further provided.

FIELD OF INVENTION

This invention relates generally to the field of soybean breeding,specifically relating to a soybean variety designated XR28L10.

BACKGROUND

The present invention relates to a new and distinctive soybean varietydesignated XR28L10, which has been the result of years of carefulbreeding and selection in a comprehensive soybean breeding program.There are numerous steps in the development of any novel, desirablesoybean variety. Plant breeding begins with the analysis and definitionof problems and weaknesses of the current germplasm, the establishmentof program goals, and the definition of specific breeding objectives.The next step is selection of germplasm that possess the traits to meetthe program goals. The breeder's goal is to combine in a single varietyan improved combination of desirable traits from the parental germplasm.These important traits may include but are not limited to: higher seedyield, resistance to diseases and insects, tolerance to drought andheat, altered fatty acid profile, abiotic stress tolerance, improvementsin compositional traits and better agronomic characteristics.

These product development processes, which lead to the final step ofmarketing and distribution, can take from six to twelve years from thetime the first cross is made until the finished seed is delivered to thefarmer for planting. Therefore, development of new varieties is atime-consuming process that requires precise planning, efficient use ofresources, and a minimum of changes in direction.

Soybean (Glycine max), is an important and valuable field crop. Thus, acontinuing goal of soybean breeders is to develop stable, high yieldingsoybean varieties that are agronomically sound. The reasons for thisgoal are to maximize the amount of grain produced on the land used andto supply food for both animals and humans. To accomplish this goal, thesoybean breeder must select and develop soybean plants that have thetraits that result in superior varieties.

The soybean is the world's leading source of vegetable oil and proteinmeal. The oil extracted from soybeans is used for cooking oil,margarine, and salad dressings. Soybean oil is composed of saturated,monounsaturated and polyunsaturated fatty acids. It has a typicalcomposition of 11% palmitic, 4% stearic, 25% oleic, 50% linoleic and 9%linolenic fatty acid content (“Economic Implications of Modified SoybeanTraits Summary Report”, Iowa Soybean Promotion Board & American SoybeanAssociation Special Report 92S, May 1990). Changes in fatty acidcomposition for improved oxidative stability and nutrition are alsoimportant traits. Industrial uses for processed soybean oil includeingredients for paints, plastics, fibers, detergents, cosmetics, andlubricants. Soybean oil may be split, inter-esterified, sulfurized,epoxidized, polymerized, ethoxylated, or cleaved. Designing andproducing soybean oil derivatives with improved functionality,oliochemistry, is a rapidly growing field. The typical mixture oftriglycerides is usually split and separated into pure fatty acids,which are then combined with petroleum-derived alcohols or acids,nitrogen, sulfonates, chlorine, or with fatty alcohols derived from fatsand oils.

Soybean is also used as a food source for both animals and humans.Soybean is widely used as a source of protein for animal feeds forpoultry, swine and cattle. During processing of whole soybeans, thefibrous hull is removed and the oil is extracted. The remaining soybeanmeal is a combination of carbohydrates and approximately 50% protein.

For human consumption soybean meal is made into soybean flour which isprocessed to protein concentrates used for meat extenders or specialtypet foods. Production of edible protein ingredients from soybean offersa healthy, less expensive replacement for animal protein in meats aswell as dairy-type products.

SUMMARY

A novel soybean variety, designated XR28L10 is provided. Also providedare the seeds of soybean variety XR28L10, cells from soybean varietyXR28L10, plants of soybean XR28L10, and plant parts of soybean varietyXR28L10. Methods provided include producing a soybean plant by crossingsoybean variety XR28L10 with another soybean plant, methods forintrogressing a transgenic, a mutant trait, and/or a native trait intosoybean variety XR28L10, methods for producing other soybean varietiesor plant parts derived from soybean variety XR28L10. Soybean seed,cells, plants, germplasm, breeding lines, varieties, and plant partsproduced by these methods and/or derived from soybean variety XR28L10are further provided.

DEFINITIONS

Certain definitions used in the specification are provided below. Alsoin the examples which follow, a number of terms are used. In order toprovide a clear and consistent understanding of the specification andclaims, the following definitions are provided:

AERIAL WEB BLIGHT. Aerial blight is caused by the fungus Rhizoctoniasolani, which can also cause seedling blight and root rot. Stems,flowers, pods, petioles, and leaves are susceptible to formation oflesions. Tolerance to Aerial Web Blight is rated on a scale of 1 to 9,with a score of 1 being very susceptible, ranging up to a score of 9being tolerant.

ALLELE. Any of one or more alternative forms of a genetic sequence. In adiploid cell or organism, the two alleles of a given sequence typicallyoccupy corresponding loci on a pair of homologous chromosomes.

ANTHESIS. The time of a flower's opening.

APHID ANTIBIOSIS. Aphid antibiosis is the ability of a variety to reducethe survival, growth, or reproduction of aphids that feed on it.Screening scores are based on the ability of the plant to decrease therate of aphid reproduction. Plants are compared to resistant andsusceptible check plants grown in the same experiment. Scores of1=susceptible, 3=below average, 5=average, 7=above average, and9=exceptional tolerance.

BACKCROSSING. Process in which a breeder crosses a donor parent varietypossessing a desired trait or traits to a recurrent parent variety(which is agronomically superior but lacks the desired level or presenceof one or more traits) and then crosses the resultant progeny back tothe recurrent parent one or more times. Backcrossing can be used tointroduce one or more desired traits from one genetic background intoanother background that is lacking the desired traits.

BREEDING. The genetic manipulation of living organisms. BU/A=Bushels perAcre. The seed yield in bushels/acre is the actual yield of the grain atharvest.

Brown Stem Rot=BSR=Brown Stem Rot Tolerance. This is a visual diseasescore from 1 to 9 comparing all genotypes in a given test. The score isbased on leaf symptoms of yellowing, necrosis and on inner stem rottingcaused by Phialophora gregata. A score of 1 indicates severe symptoms ofleaf yellowing and necrosis. Increasing visual scores from 2 to 8indicate additional levels of tolerance, while a score of 9 indicates nosymptoms.

BSRLF=Brown Stem Rot disease rating based solely on leaf diseasesymptoms. This is a visual disease score from 1 to 9 comparing allgenotypes in a given test. A score of 1 indicates severe leaf yellowingand necrosis Increasing visual scores from 2 to 8 indicate additionallevels of tolerance, while a score of 9 indicates no leaf symptoms

BSRSTM=Brown Stem Rot disease rating based solely on stem diseasesymptoms. This is a visual disease score from 1 to 9 comparing allgenotypes in a given test. A score of 1 indicates severe necrosis on theinner stem tissues. Increasing visual scores from 2 to 8 indicateadditional levels of tolerance, while a score of 9 indicates no innerstem symptoms

CELL. Cell as used herein includes a plant cell, whether isolated, intissue culture, or incorporated in a plant or plant part.

CHARCOAL ROT DISEASE. A fungal disease caused by Macrophomina phaseolinathat is enhanced by hot and dry conditions, especially duringreproductive growth stages. Tolerance score is based on observations ofthe comparative ability to tolerate drought and limit losses fromcharcoal rot infection among various soybean varieties. A score of 1indicates severe charcoal rot on the roots and dark microsclerotia onthe lower stem. Increasing visual scores from 2 to 8 indicate additionallevels of tolerance, while a score of 9 indicates no lower stem and/orroot rot.

CHLORIDE SENSITIVITY. This is a measure of the chloride concentration inthe plant tissue from 1 to 9. The higher the score the lower theconcentration of chloride in the tissue measured.

CW or Canopy Width. This is a visual observation of the canopy widthwhich is scored from 1 to 9 comparing all genotypes in a given test. Ascore of 1=very narrow, while a score of 9=very bushy.

CNKR or Stem Canker Tolerance. This is a visual disease score from 1 to9 comparing all genotypes in a given field test. The score is based uponfield reaction to the disease. A score of 1 indicates susceptibility tothe disease, whereas a score of 9 indicates the line is resistant to thedisease.

STEM CANKER GENE. Resistance based on a specific gene that infersspecific resistance or susceptibility to a specific race of Stem Canker.The score is based upon a reaction of tooth pick inoculation with a raceof stem canker. A score of 1 indicates severe stem canker lesions,similar to a known susceptible check variety, whereas a score of 9indicates no disease symptoms, consistent with a known resistant checkvariety

COTYLEDON. A cotyledon is a type of seed leaf. The cotyledon containsthe food storage tissues of the seed.

CROSS-POLLINATION. Fertilization by the union of two gametes fromdifferent plants.

DIPLOID. A cell or organism having two sets of chromosomes.

ELITE VARIETY. A variety that is sufficiently homozygous and homogeneousto be used for commercial grain production. An elite variety may also beused in further breeding.

EMBRYO. The embryo is the small plant contained within a mature seed.

EMGSC=Field Emergence=Emergence Score. A score based upon speed andstrength of emergence at sub-optimal conditions. Rating is done at theunifoliate to first trifoliate stages of growth. A score using a 1 to 9scale is given, with 1 being the poorest and 9 the best. Scores of 1, 2,and 3=degrees of unacceptable stands; slow growth and poor plant health.Scores of 4, 5, 6=degrees of less than optimal stands; moderate growthand plant health. Scores of 7, 8, 9,=degrees of optimal stands; vigorousgrowth and plant health.

FEC=Iron-deficiency Chlorosis=Iron Chlorosis. Plants are scored 1 to 9based on visual observations. A score of 1 indicates the plants are deador dying from iron-deficiency chlorosis, a score of 5 means plants haveintermediate health with some leaf yellowing, and a score of 9 means nostunting of the plants or yellowing of the leaves. Plots are usuallyscored in mid July.

FECL=Iron-deficiency Chlorosis-Late. Plants are scored 1 to 9 based onvisual observations. A score of 1 indicates the plants are dead or dyingfrom iron-deficiency chlorosis, a score of 5 means plants haveintermediate health with some leaf yellowing and a score of 9 means nostunting of the plants or yellowing of the leaves. Plots are scoredlater in the growing season, typically around mid August.

FEY or Frogeye Leaf Spot. This is a visual fungal disease score from 1to 9 comparing all genotypes in a given experiment. The score is basedupon the number and size of leaf lesions. A score of 1 indicates severeleaf necrosis spotting, whereas a score of 9 indicates no lesions.

FLOWER COLOR. Data values include: P=purple and W=white.

GENE SILENCING. The interruption or suppression of the expression of anucleic acid sequence at the level of transcription or translation.

GENOTYPE. Refers to the genetic constitution of a cell or organism.

PLANT HABIT. This refers to the physical appearance of a plant. It canbe determinate (Det), semi-determinate, intermediate, or indeterminate(Ind). In soybeans, indeterminate varieties are those in which stemgrowth is not limited by formation of a reproductive structure (i.e.,flowers, pods and seeds) and hence growth continues throughout floweringand during part of pod filling. The main stem will develop and set podsover a prolonged period under favorable conditions. In soybeans,determinate varieties are those in which stem growth ceases at floweringtime. Most flowers develop simultaneously, and most pods fill atapproximately the same time. The terms semi-determinate and intermediateare also used to describe plant habit and are defined in Bernard, R. L.1972. “Two genes affecting stem termination in soybeans.” Crop Science12:235-239; Woodworth, C. M. 1932. “Genetics and breeding in theimprovement of the soybean.” Bull. Agric. Exp. Stn. (Illinois)384:297-404; Woodworth, C. M. 1933. “Genetics of the soybean.” J. Am.Soc. Agron. 25:36-51.

HAPLOID. A cell or organism having one set of the two sets ofchromosomes in a diploid cell or organism.

HERBRES=Herbicide Resistance. This indicates that the plant is moretolerant to the herbicide shown than the level of herbicide toleranceexhibited by wild type plants. A designation of RR indicates toleranceto glyphosate and a designation of STS indicates tolerance tosulfonylurea herbicides.

HGT=Plant Height. Plant height is taken from the top of the soil to thetop pod of the plant and is measured in inches.

HILUM. This refers to the scar left on the seed which marks the placewhere the seed was attached to the pod prior to harvest. Hila Color datavalues include: BR=brown; TN=tan; Y=yellow; BL=black; IB=ImperfectBlack; BF=buff.

HYPL=Hypocotyl length=Hypocotyl elongation. This score indicates theability of the seed to emerge when planted 3″ deep in sand pots and witha controlled temperature of 25° C. The number of plants that emerge eachday are counted. Based on this data, each genotype is given a score from1 to 9 based on its rate of emergence and the percent of emergence. Ascore of 1 indicates a very poor rate and percent of emergence, anintermediate score of 5 indicates average ratings, and a score of 9indicates an excellent rate and percent of emergence

HYPOCOTYL. A hypocotyl is the portion of an embryo or seedling betweenthe cotyledons and the root.

LDGSEV=Lodging Resistance=Harvest Standability. Lodging is rated on ascale of 1 to 9. A score of 1 indicates plants that are laying on theground, a score of 5 indicates plants are leaning at a 45° angle inrelation to the ground, and a score of 9 indicates erect plants.

LEAFLETS. These are part of the plant shoot, and they manufacture foodfor the plant by the process of photosynthesis.

LINKAGE. Refers to a phenomenon wherein alleles on the same chromosometend to segregate together more often than expected by chance if theirtransmission was independent.

LINKAGE DISEQUILIBRIUM. Refers to a phenomenon wherein alleles tend toremain together in linkage groups when segregating from parents tooffspring, with a greater frequency than expected from their individualfrequencies.

LLC=Oil with three percent or less linolenic acid is classified as lowlinolenic oil. Linolenic acid is one of the five most abundant fattyacids in soybean seeds. It is measured by gas chromatography and isreported as a percent of the total oil content.

LLE=Linoleic Acid Percent. Linoleic acid is one of the five mostabundant fatty acids in soybean seeds. It is measured by gaschromatography and is reported as a percent of the total oil content.

LLN=Linolenic Acid Percent. Linolenic acid is one of the five mostabundant fatty acids in soybean seeds. It is measured by gaschromatography and is reported as a percent of the total oil content.

LOCUS. A defined segment of DNA.

PRM Predicted Relative Maturity or Relative Maturity. Soybean maturitiesare divided into relative maturity groups (00, 0, I, II, III, IV . . . Xor 00, 0, 1, 2, 3, . . . 10). Within a maturity group are sub-groups. Asub-group is a tenth of a relative maturity group (for example 1.3 wouldindicate a group 1 and subgroup 3).

MAT ABS=Absolute Maturity. This term is defined as the length of timefrom planting to complete physiological development (maturity). Theperiod from planting until maturity is reached is measured in days,usually in comparison to one or more standard varieties. Plants areconsidered mature when 95% of the pods have reached their mature color.

MATURITY GROUP. This refers to an agreed-on industry division of groupsof varieties, based on the zones in which they are adapted primarilyaccording to day length or latitude. They consist of very long daylength varieties (Groups 000, 00, 0), and extend to very short daylength varieties (Groups VII, VIII, IX, X).

Narrow rows. Term indicates 7″ and 15″ row spacing.

NEI DISTANCE. A quantitative measure of percent similarity between twolines. Nei's distance between lines A and B can be defined as1−((2*number alleles in common)/(number alleles in A+number alleles inB)). For example, if lines A and B are the same for 95 out of 100alleles, the Nei distance would be 0.05. If lines A and B are the samefor 98 out of 100 alleles, the Nei distance would be 0.02. Free softwarefor calculating Nei distance is available on the internet at multiplelocations such as, for example, at:evolution.genetics.washington.edu/phylip.html. See Nei & Li (1979) ProcNatl Acad Sci USA 76:5269-5273, which is incorporated by reference forthis purpose.

NUCLEIC ACID. An acidic, chainlike biological macromolecule consistingof multiple repeat units of phosphoric acid, sugar and purine andpyrimidine bases.

OIL=Oil Percent. Soybean seeds contain a considerable amount of oil. Oilis measured by NIR spectrophotometry, and is reported as a percentagebasis.

Oil/Meal TYPE: Designates varieties specially developed with thefollowing oil traits: HLC=High Oleic oil; LLC=Low Linolenic (3%linolenic content); ULC=Ultra Low Linolenic oil (1% linolenic oilcontent); HSC=High Sucrose meal; LPA=Low Phytic Acid; LST=Low Saturateoil; Blank=Conventional variety/oil composition.

OLC=Oleic Acid Percent. Oleic acid is one of the five most abundantfatty acids in soybean seeds. It is measured by gas chromatography andis reported as a percent of the total oil content.

PEDIGREE DISTANCE. Relationship among generations based on theirancestral links as evidenced in pedigrees. May be measured by thedistance of the pedigree from a given starting point in the ancestry.

PERCENT IDENTITY. Percent identity as used herein refers to thecomparison of the homozygous alleles of two soybean varieties. Percentidentity is determined by comparing a statistically significant numberof the homozygous alleles of two developed varieties. For example, apercent identity of 90% between soybean variety 1 and soybean variety 2means that the two varieties have the same allele at 90% of the lociused in the comparison.

PERCENT SIMILARITY. Percent similarity as used herein refers to thecomparison of the homozygous alleles of a soybean variety such asXR28L10 with another plant, and if the homozygous allele of XR28L10matches at least one of the alleles from the other plant then they arescored as similar. Percent similarity is determined by comparing astatistically significant number of loci and recording the number ofloci with similar alleles as a percentage. A percent similarity of 90%between XR28L10 and another plant means that XR28L10 matches at leastone of the alleles of the other plant at 90% of the loci used in thecomparison.

PLANT. As used herein, the term “plant” includes reference to animmature or mature whole plant, including a plant from which seed orgrain or anthers have been removed. Seed or embryo that will produce theplant is also considered to be the plant.

PLANT PARTS. As used herein, the term “plant parts” includes leaves,stems, roots, root tips, anthers, seed, grain, embryo, pollen, ovules,flowers, cotyledon, hypocotyl, pod, flower, shoot, stalk, tissue, cellsand the like.

PLM or Palmitic Acid Percent. Palmitic acid is one of the five mostabundant fatty acids in soybean seeds. It is measured by gaschromatography and is reported as a percent of the total oil content.

PMG infested soils: soils containing Phytophthora sojae.

POD. This refers to the fruit of a soybean plant. It consists of thehull or shell (pericarp) and the soybean seeds. Pod Color data valuesinclude: BR=brown; TN=tan.

PRT or Phytophthora Field Tolerance. Tolerance to Phytophthora root rotis rated on a scale of 1 to 9, with a score of 1 indicating the plantshave no tolerance to Phytophthora, ranging to a score of 9 being thebest or highest tolerance.

PHYTOPHTHORA RESISTANCE GENE (Rps). Various Phytophthora resistancegenes are known and include: Rps1a=resistance to races 1-2, 10-11, 13-8,24; Rps1c=resistance to races 1-3,6-11, 13, 15, 17, 21, 23, 24, 26,28-30, 32, 34, 36; Rps1k=resistance to races 1-11, 13-15, 17, 18, 21-24,26, 36, 37; Rps6=resistance to races 1-4, 10, 12, 14-16, 18-21, 25, 28,33-35; and (−) indicates no specific gene for resistance is detected.

PRMMAT or Predicted Relative Maturity. Soybean maturities are dividedinto relative maturity groups. In the United States the most commonmaturity groups are 00 through VIII. Within maturity groups 00 through Vare sub-groups. A sub-group is a tenth of a relative maturity group.Within narrow comparisons, the difference of a tenth of a relativematurity group equates very roughly to a day difference in maturity atharvest.

PRO or Protein Percent. Soybean seeds contain a considerable amount ofprotein. Protein is generally measured by NIR spectrophotometry, and isreported on a dry weight basis.

PUBESCENCE. This refers to a covering of very fine hairs closelyarranged on the leaves, stems and pods of the soybean plant. PubescenceColor-data values include: L=Light Tawny; T=Tawny; G=Gray.

R160 or Palmitic Acid percentage. Percentage of palmitic acid asdetermined using methods described in Reske, et al., TriacylglycerolComposition and Structure in Genetically Modified Sunflower and SoybeanOils. JAOCS 74:8, 989-998 (1997), which is incorporated by reference forthis purpose.

R180 or Stearic acid percentage. Percentage of Stearic acid asdetermined using methods described in Reske, et al., TriacylglycerolComposition and Structure in Genetically Modified Sunflower and SoybeanOils. JAOCS 74:8, 989-998 (1997), which is incorporated by reference forthis purpose.

R181 or Oleic acid percentage. Percentage of oleic acid as determinedusing methods described in Reske, et al., Triacylglycerol Compositionand Structure in Genetically Modified Sunflower and Soybean Oils. JAOCS74:8, 989-998 (1997), which is incorporated by reference for thispurpose.

R182 or Linoleic acid percentage. Percentage of linoleic acid asdetermined using methods described in Reske, et al., TriacylglycerolComposition and Structure in Genetically Modified Sunflower and SoybeanOils. JAOCS 74:8, 989-998 (1997), which is incorporated by reference forthis purpose.

R183 or Linolenic acid percentage. Percentage of linolenic acid asdetermined using methods described in Reske, et al., TriacylglycerolComposition and Structure in Genetically Modified Sunflower and SoybeanOils. JAOCS 74:8, 989-998 (1997), which is incorporated by reference forthis purpose.

RESISTANCE. Synonymous with tolerance. The ability of a plant towithstand exposure to an insect, disease, herbicide, environmentalstress, or other condition. A resistant plant variety will be able tobetter withstand the insect, disease pathogen, herbicide, environmentalstress, or other condition as compared to a non-resistant or wild-typevariety.

RKI or Root-knot Nematode, Southern. This is a visual disease score from1 to 9 comparing all genotypes in a given experiment. The score isdetermined by digging plants to visually score the roots for presence orabsence of galling. A score of 1 indicates large severe galling coveringmost of the root system which results in pre-mature death fromdecomposition of the root system. A score of 9 indicates that there isno galling of the roots.

RKA or Root-knot Nematode, Peanut. This is a visual disease score from 1to 9 comparing all genotypes in a given experiment. The score isdetermined by digging plants to score the roots for presence or absenceof galling. A score of 1 indicates large severe galling covering most ofthe root system which results in pre-mature death from decomposition ofthe root system. A score of 9 indicates that there is no galling of theroots.

SCN=Soybean Cyst Nematode Resistance=Cyst Nematode Resistance. The scoreis based on resistance to a particular race of soybean cyst nematode,such as race 1, 2, 3, 5 or 14. Scores are from 1 to 9 and indicatevisual observations of resistance as compared to other genotypes in thetest. A score of 1 indicates nematodes are able to infect the plant andcause yield loss, while a score of 9 indicates SCN resistance.

SCN Resistance Source. There are three typical sources of geneticresistance to SCN: PI88788, PI548402 (also known as Peking), andPI437654 (also known as Hartwig).

SCN infected soils: soils containing soybean cyst nematode.

SD VIG or Seedling Vigor. The score is based on the speed of emergenceof the plants within a plot relative to other plots within anexperiment. A score of 1 indicates no plants have expanded first leaves,while a score of 9 indicates that 90% of plants growing have expandedfirst leaves.

SDS or Sudden Death Syndrome is caused by the fungal pathogen Fusariumsolani f.sp. glycines. Tolerance to Sudden Death Syndrome is rated on ascale of 1 to 9, with a score of 1 being very susceptible ranging up toa score of 9 being tolerant.

SEED COAT LUSTER. Data values include D=dull; S=shiny.

SEED SIZE SCORE. This is a measure of the seed size from 1 to 9. Thehigher the score the smaller the seed size measured.

SPLB=S/LB=Seeds per Pound. Soybean seeds vary in seed size, therefore,the number of seeds required to make up one pound also varies. Thisaffects the pounds of seed required to plant a given area, and can alsoimpact end uses.

SHATTR or Shattering. This refers to the amount of pod dehiscence priorto harvest. Pod dehiscence involves seeds falling from the pods to thesoil. This is a visual score from 1 to 9 comparing all genotypes withina given test. A score of 1 indicates 100% of the pods are opened, whilea score of 9 means pods have not opened and no seeds have fallen out.

SHOOTS. These are a portion of the body of the plant. They consist ofstems, petioles and leaves.

STC or Stearic Acid Percent. Stearic acid is one of the five mostabundant fatty acids in soybean seeds. It is measured by gaschromatography and is reported as a percent of the total oil content.

SUBLINE. Although XR28L10 contains substantially fixed genetics, and isphenotypically uniform and with no off-types expected, there stillremains a small proportion of segregating loci either within individualsor within the population as a whole.

WHMD or White Mold Tolerance. This is a fungal disease caused bySclerotinia sclerotiorum that creates mycelial growth and death ofplants. Tolerance to white mold is scored from 1 to 9 by visuallycomparing all genotypes in a given test. A score of 1 indicates completedeath of the experimental unit while a score of 9 indicates no symptoms.

VARIETY. A substantially homozygous soybean line and minor modificationsthereof that retain the overall genetics of the soybean line includingbut not limited to a subline, a locus conversion, a mutation, atransgenic, or a somaclonal variant.

High yield environments. Areas which lack normal stress, typicallyhaving sufficient rainfall, water drainage, low disease pressure, andlow weed pressure

Tough environments. Areas which have stress challenges, opposite of ahigh yield environment

DETAILED DESCRIPTION

The variety has shown uniformity and stability for all traits, asdescribed in the following variety description information. It has beenself-pollinated a sufficient number of generations, with carefulattention to uniformity of plant type to ensure a sufficient level ofhomozygosity and phenotypic stability. The variety has been increasedwith continued observation for uniformity. No variant traits have beenobserved or are expected.

A variety description of Soybean variety XR28L10 is provided in Table 1.Traits reported are average values for all locations and years orsamples measured.

Soybean variety XR28L10, being substantially homozygous, can bereproduced by planting seeds of the variety, growing the resultingsoybean plants under self-pollinating or sib-pollinating conditions, andharvesting the resulting seed, using techniques familiar to theagricultural arts.

Performance Examples of XR28L10

As shown in Table 2, the traits and characteristics of soybean varietyXR28L10 are given in paired comparisons with other varieties. Traitsreported are mean values for all locations and years where pairedcomparison data was obtained.

Genetic Marker Profile

In addition to phenotypic observations, a plant can also be identifiedby its genotype. The genotype of a plant can be characterized through agenetic marker profile which can identify plants of the same variety ora related variety, or which can be used to determine or validate apedigree. Genetic marker profiles can be obtained by techniques such asrestriction fragment length polymorphisms (RFLPs), randomly amplifiedpolymorphic DNAs (RAPDs), arbitrarily primed polymerase chain reaction(AP-PCR), DNA amplification fingerprinting (DAF), sequence characterizedamplified regions (SCARs), amplified fragment length polymorphisms(AFLPs), simple sequence repeats (SSRs) also referred to asmicrosatellites, or single nucleotide polymorphisms (SNPs). For example,see Cregan et al, “An Integrated Genetic Linkage Map of the SoybeanGenome” Crop Science 39:1464-1490 (1999), and Berry et al., AssessingProbability of Ancestry Using Simple Sequence Repeat Profiles:Applications to Maize Inbred Lines and Soybean Varieties” Genetics165:331-342 (2003), each of which are incorporated by reference hereinin their entirety.

Particular markers used for these purposes are not limited to anyparticular set of markers, but are envisioned to include any type ofmarker and marker profile which provides a means of distinguishingvarieties. One method of comparison is to use only homozygous loci forXR28L10. For example, one set of publicly available markers which couldbe used to screen and identify variety XR28L10 is disclosed in Table 3.

Primers and PCR protocols for assaying these and other markers aredisclosed in Soybase (sponsored by the USDA Agricultural ResearchService and Iowa State University) located at the world wide web at129.186.26.94/SSR.html. In addition to being used for identification ofsoybean variety XR28L10 and plant parts and plant cells of varietyXR28L10, the genetic profile may be used to identify a soybean plantproduced through the use of XR28L10 or to verify a pedigree for progenyplants produced through the use of XR28L10. The genetic marker profileis also useful in breeding and developing backcross conversions.

The present invention comprises a soybean plant characterized bymolecular and physiological data obtained from the representative sampleof said variety deposited with the ATCC. Further provided is a soybeanplant formed by the combination of the disclosed soybean plant or plantcell with another soybean plant or cell and comprising the homozygousalleles of the variety.

Means of performing genetic marker profiles using SSR polymorphisms arewell known in the art. A marker system based on SSRs can be highlyinformative in linkage analysis relative to other marker systems in thatmultiple alleles may be present. Another advantage of this type ofmarker is that, through use of flanking primers, detection of SSRs canbe achieved, for example, by using the polymerase chain reaction (PCR),thereby eliminating the need for labor-intensive Southern hybridization.PCR detection is done using two oligonucleotide primers flanking thepolymorphic segment of repetitive DNA to amplify the SSR region.

Following amplification, markers can be scored by electrophoresis of theamplification products. Scoring of marker genotype is based on the sizeof the amplified fragment, which correlates to the number of base pairsof the fragment. While variation in the primer used or in laboratoryprocedures can affect the reported fragment size, relative values shouldremain constant regardless of the specific primer or laboratory used.When comparing varieties it is preferable if all SSR profiles areperformed in the same lab.

Primers used are publicly available and may be found in Soybase orCregan (1999 Crop Science 39:1464-1490). See also, WO 99/31964Nucleotide Polymorphisms in Soybean, U.S. Pat. No. 6,162,967 PositionalCloning of Soybean Cyst Nematode Resistance Genes, and U.S. Pat. No.7,288,386 Soybean Sudden Death Syndrome Resistant Soybeans and Methodsof Breeding and Identifying Resistant Plants, the disclosures of whichare incorporated herein by reference.

The SSR profile of soybean plant XR28L10 can be used to identify plantscomprising XR28L10 as a parent, since such plants will comprise the samehomozygous alleles as XR28L10. Because the soybean variety isessentially homozygous at all relevant loci, most loci should have onlyone type of allele present. In contrast, a genetic marker profile of anF1 progeny should be the sum of those parents, e.g., if one parent washomozygous for allele x at a particular locus, and the other parenthomozygous for allele y at that locus, then the F1 progeny will be xy(heterozygous) at that locus. Subsequent generations of progeny producedby selection and breeding are expected to be of genotype xx(homozygous), yy (homozygous), or xy (heterozygous) for that locusposition. When the F1 plant is selfed or sibbed for successive filialgenerations, the locus should be either x or y for that position.

In addition, plants and plant parts substantially benefiting from theuse of XR28L10 in their development, such as XR28L10 comprising abackcross conversion, transgene, or genetic sterility factor, may beidentified by having a molecular marker profile with a high percentidentity to XR28L10. Such a percent identity might be 90%, 95%, 96%,97%, 98%, 99%, 99.5% or 99.9% identical to XR28L10.

The SSR profile of XR28L10 also can be used to identify essentiallyderived varieties and other progeny varieties developed from the use ofXR28L10, as well as cells and other plant parts thereof. Such plants maybe developed using the markers identified in WO 00/31964, U.S. Pat. No.6,162,967 and U.S. Pat. No. 7,288,386. Progeny plants and plant partsproduced using XR28L10 may be identified by having a molecular markerprofile of at least 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%,75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%,89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 99.5% geneticcontribution from soybean variety, as measured by either percentidentity or percent similarity. Such progeny may be furthercharacterized as being within a pedigree distance of XR28L10, such aswithin 1, 2, 3, 4 or 5 or less cross-pollinations to a soybean plantother than XR28L10, or a plant that has XR28L10 as a progenitor. Uniquemolecular profiles may be identified with other molecular tools such asSNPs and RFLPs.

Introduction of a New Trait or Locus into XR28L10

Variety XR28L10 represents a new base genetic variety into which a newlocus or trait may be introgressed. Direct transformation andbackcrossing represent two important methods that can be used toaccomplish such an introgression.

A backcross conversion of XR28L10 occurs when DNA sequences areintroduced through backcrossing (Hallauer et al. in Corn and CornImprovement, Sprague and Dudley, Third Ed. 1998), with XR28L10 utilizedas the recurrent parent. Both naturally occurring and transgenic DNAsequences may be introduced through backcrossing techniques. A backcrossconversion may produce a plant with a trait or locus conversion in atleast two or more backcrosses, including at least 2 backcrosses, atleast 3 backcrosses, at least 4 backcrosses, at least 5 backcrosses, ormore. Molecular marker assisted breeding or selection may be utilized toreduce the number of backcrosses necessary to achieve the backcrossconversion. For example, see Openshaw, S. J. et al., Marker-assistedSelection in Backcross Breeding. In: Proceedings Symposium of theAnalysis of Molecular Data, August 1994, Crop Science Society ofAmerica, Corvallis, Oreg., where it is demonstrated that a backcrossconversion can be made in as few as two backcrosses.

The complexity of the backcross conversion method depends on the type oftrait being transferred (a single gene or closely linked genes comparedto unlinked genes), the level of expression of the trait, the type ofinheritance (cytoplasmic or nuclear), dominant or recessive traitexpression, and the types of parents included in the cross. It isunderstood by those of ordinary skill in the art that for single genetraits that are relatively easy to classify, the backcross method iseffective and relatively easy to manage. (See Hallauer et al. in Cornand Corn Improvement, Sprague and Dudley, Third Ed. 1998). Desiredtraits that may be transferred through backcross conversion include, butare not limited to, sterility (nuclear and cytoplasmic), fertilityrestoration, nutritional enhancements, drought tolerance, nitrogenutilization, altered fatty acid profile, low phytate, industrialenhancements, disease resistance (bacterial, fungal or viral), insectresistance, and herbicide resistance. In addition, a recombination siteitself, such as an FRT site, Lox site or other site specific integrationsite, may be inserted by backcrossing and utilized for direct insertionof one or more genes of interest into a specific plant variety. A singlelocus may contain several transgenes, such as a transgene for diseaseresistance that also contains a transgene for herbicide resistance. Thegene for herbicide resistance may be used as a selectable marker and/oras a phenotypic trait. A single locus conversion of site specificintegration system allows for the integration of multiple genes at aknown recombination site in the genome.

The backcross conversion may result from either the transfer of adominant allele or a recessive allele. Selection of progeny containingthe trait of interest is accomplished by direct selection for a traitassociated with a dominant allele. Transgenes transferred viabackcrossing typically function as a dominant single gene trait and arerelatively easy to classify. Selection of progeny for a trait that istransferred via a recessive allele requires growing and selfing thefirst backcross generation to determine which plants carry the recessivealleles. Recessive traits may require additional progeny testing insuccessive backcross generations to determine the presence of the locusof interest. The last backcross generation is usually selfed to givepure breeding progeny for the trait(s) being transferred, although abackcross conversion with a stably introgressed trait may also bemaintained by further backcrossing to the recurrent parent withsubsequent selection for the trait.

Along with selection for the trait of interest, progeny are selected forthe phenotype of the recurrent parent. The backcross is a form ofinbreeding, and the features of the recurrent parent are automaticallyrecovered after successive backcrosses. Poehlman suggests from one tofour or more backcrosses, but as noted above, the number of backcrossesnecessary can be reduced with the use of molecular markers (Poehlman etal (1995) Breeding Field Crops, 4th Ed., Iowa State University Press,Ames, Iowa). Other factors, such as a genetically similar donor parent,may also reduce the number of backcrosses necessary. As noted byPoehlman, backcrossing is easiest for simply inherited, dominant andeasily recognized traits.

One process for adding or modifying a trait or locus in soybean varietyXR28L10 comprises crossing XR28L10 plants grown from XR28L10 seed withplants of another soybean plant that comprises a desired trait lackingin XR28L10, selecting F1 progeny plants that possess the desired traitor locus to produce selected F1 progeny plants, crossing the selectedprogeny plants back to XR28L10 plants to produce backcross1 (BC1)progeny plants. The BC1F1 progeny plants that have the desired trait andthe morphological characteristics of soybean variety XR28L10 areselected and backcrossed to XR28L10 to generate BC2F1 progeny plants.Additional backcrossing and selection of progeny plants with the desiredtrait will produce BC3F1, BC4F1, BC5F1, . . . BCxF1 generations ofplants. The backcross populations of XR28L10 may be furthercharacterized as having the physiological and morphologicalcharacteristics of soybean variety XR28L10 listed in Table 1 asdetermined at the 5% significance level when grown in the sameenvironmental conditions and/or may be characterized by percentsimilarity or identity to XR28L10 as determined by SSR or othermolecular markers. The above method may be utilized with fewerbackcrosses in appropriate situations, such as when the donor parent ishighly related or molecular markers are used in the selection step.Desired traits that may be used include those nucleic acids known in theart, some of which are listed herein, that will affect traits throughnucleic acid expression or inhibition. Desired loci also include theintrogression of FRT, Lox and/or other recombination sites for sitespecific integration. Desired loci further include QTLs, which may alsoaffect a desired trait.

In addition, the above process and other similar processes describedherein may be used to produce first generation progeny soybean seed byadding a step at the end of the process that comprises crossing XR28L10with the introgressed trait or locus with a different soybean plant andharvesting the resultant first generation progeny soybean seed.

Transgenes and transformation methods provide means to engineer thegenome of plants to contain and express foreign genetic elements,additional copies of endogenous elements, and/or modified versions ofnative or endogenous genetic elements in order to alter the traits of aplant in a specific manner that would be difficult or impossible toobtain with traditional plant breeding alone. Any heterologous DNAsequence(s), whether from a different species or from the same species,which are inserted into the genome using transformation, backcrossing orother methods known to one of skill in the art are referred to hereincollectively as transgenes. The sequences are heterologous based onsequence source, location of integration, operably linked elements, orany combination thereof. Transgenic variants of soybean variety XR28L10,seeds, cells, and parts thereof or derived therefrom are provided.

In one example a process for modifying soybean variety XR28L10 with theaddition of a desired trait, said process comprising transforming asoybean plant of variety XR28L10 with a transgene that confers a desiredtrait is provided. Therefore, transgenic XR28L10 soybean cells, plants,plant parts, and seeds produced from this process are provided. In someexamples, the desired trait may be one or more of herbicide resistance,insect resistance, disease resistance, decreased phytate, modified fattyacid profile, modified fatty acid content, or carbohydrate metabolism.The specific gene may be any known in the art or listed herein,including but not limited to a polynucleotide conferring resistance toimidazolinone, sulfonylurea, glyphosate, glufosinate, triazine orbenzonitrile herbicides; a polynucleotide encoding a Bacillusthuringiensis polypeptide, a polynucleotide encoding a phytase, a fattyacid desaturase (e.g., FAD-2, FAD-3), galactinol synthase, a raffinosesynthetic enzyme; or a polynucleotide conferring resistance to soybeancyst nematode, brown stem rot, Phytophthora root rot, soybean mosaicvirus, sudden death syndrome, or other plant pathogen.

Numerous methods for plant transformation have been developed, includingbiological and physical plant transformation protocols. See, forexample, Miki et al., “Procedures for Introducing Foreign DNA intoPlants” in Methods in Plant Molecular Biology and Biotechnology, Glick,B. R. and Thompson, J. E. Eds. (CRC Press, Inc., Boca Raton, 1993) pages67-88; and Armstrong, “The First Decade of Maize Transformation: AReview and Future Perspective” (Maydica 44:101-109, 1999). In addition,expression vectors and in vitro culture methods for plant cell or tissuetransformation and regeneration of plants are available. See, forexample, Gruber et al., “Vectors for Plant Transformation” in Methods inPlant Molecular Biology and Biotechnology, Glick, B. R. and Thompson, J.E. Eds. (CRC Press, Inc., Boca Raton, 1993) pages 89-119.

The most prevalent types of plant transformation involve theconstruction of an expression vector. Such a vector comprises a DNAsequence that contains a gene under the control of or operatively linkedto a regulatory element, for example a promoter. The vector may containone or more genes and one or more regulatory elements.

A genetic trait which has been engineered into the genome of aparticular soybean plant may then be moved into the genome of anothervariety using traditional breeding techniques that are well known in theplant breeding arts. For example, a backcrossing approach is commonlyused to move a transgene from a transformed soybean variety into anelite soybean variety, and the resulting backcross conversion plantwould then contain the transgene(s).

Various genetic elements can be introduced into the plant genome usingtransformation. These elements include, but are not limited to genes;coding sequences; inducible, constitutive, and tissue specificpromoters; enhancing sequences; and signal and targeting sequences.

Transgenic plants can be used to produce commercial quantities of aforeign protein. Thus, techniques for the selection and propagation oftransformed plants, which are well understood in the art, yield aplurality of transgenic plants that are harvested in a conventionalmanner, and a foreign protein then can be extracted from a tissue ofinterest or from total biomass. Protein extraction from plant biomasscan be accomplished by known methods which are discussed, for example,by Heney and Orr, Anal. Biochem. 114:92-6 (1981).

A genetic map can be generated, primarily via conventional restrictionfragment length polymorphisms (RFLP), polymerase chain reaction (PCR)analysis, simple sequence repeats (SSR) and single nucleotidepolymorphisms (SNP) that identifies the approximate chromosomal locationof the integrated DNA molecule. For exemplary methodologies in thisregard, see Glick and Thompson, Methods in Plant Molecular Biology andBiotechnology, pp. 269-284 (CRC Press, Boca Raton, 1993).

Wang et al. discuss “Large Scale Identification, Mapping and Genotypingof Single-Nucleotide Polymorphisms in the Human Genome”, Science,280:1077-1082, 1998, and similar capabilities are becoming increasinglyavailable for the soybean genome. Map information concerning chromosomallocation is useful for proprietary protection of a subject transgenicplant. If unauthorized propagation is undertaken and crosses made withother germplasm, the map of the integration region can be compared tosimilar maps for suspect plants to determine if the latter have a commonparentage with the subject plant. Map comparisons could involvehybridizations, RFLP, PCR, SSR, sequencing or combinations thereof, allof which are conventional techniques. SNPs may also be used alone or incombination with other techniques.

Likewise, plants can be genetically engineered to express variousphenotypes of agronomic interest. Through the transformation of soybeanthe expression of genes can be altered to enhance disease resistance,insect resistance, herbicide resistance, agronomic, grain quality andother traits. Transformation can also be used to insert DNA sequenceswhich control or help control male-sterility. DNA sequences native tosoybean as well as non-native DNA sequences can be transformed intosoybean and used to alter levels of native or non-native proteins.Various promoters, targeting sequences, enhancing sequences, and otherDNA sequences can be inserted into the genome for the purpose ofaltering the expression of proteins. Reduction of the activity ofspecific genes (also known as gene silencing, or gene suppression) isdesirable for several aspects of genetic engineering in plants.

Many techniques for gene silencing are well known to one of skill in theart, including but not limited to knock-outs (such as by insertion of atransposable element such as mu (Vicki Chandler, The Maize Handbook ch.118 (Springer-Verlag 1994) antisense technology (see, e.g., Sheehy etal. (1988) PNAS USA 85:8805-8809; and U.S. Pat. Nos. 5,107,065;5,453,566; and 5,759,829); co-suppression (e.g., Taylor (1997) PlantCell 9:1245; Jorgensen (1990) Trends Biotech. 8(12):340-344; Flavell(1994) PNAS USA 91:3490-3496; Finnegan et al. (1994) Bio/Technology12:883-888; and Neuhuber et al. (1994) Mol. Gen. Genet. 244:230-241);RNA interference (Napoli et al. (1990) Plant Cell 2:279-289; U.S. Pat.No. 5,034,323; Sharp (1999) Genes Dev. 13:139-141; Zamore et al. (2000)Cell 101:25-33; and Montgomery et al. (1998) PNAS USA 95:15502-15507),virus-induced gene silencing (Burton, et al. (2000) Plant Cell12:691-705; and Baulcombe (1999) Curr. Op. Plant Biol. 2:109-113);target-RNA-specific ribozymes (Haseloff et al. (1988) Nature 334:585-591); hairpin structures (Smith et al. (2000) Nature 407:319-320; WO99/53050; and WO 98/53083); microRNA (Aukerman & Sakai (2003) Plant Cell15:2730-2741); ribozymes (Steinecke et al. (1992) EMBO J. 11:1525; andPerriman et al. (1993) Antisense Res. Dev. 3:253); oligonucleotidemediated targeted modification (e.g., WO 03/076574 and WO 99/25853);Zn-finger targeted molecules (e.g., WO 01/52620; WO 03/048345; and WO00/42219); and other methods or combinations of the above methods knownto those of skill in the art.

Exemplary nucleotide sequences that may be altered by geneticengineering include, but are not limited to, those categorized below.

1. Transgenes That Confer Resistance To Insects or Disease And ThatEncode:

(A) Plant disease resistance genes. Plant defenses are often activatedby specific interaction between the product of a disease resistance gene(R) in the plant and the product of a corresponding avirulence (Avr)gene in the pathogen. A plant variety can be transformed with clonedresistance gene to engineer plants that are resistant to specificpathogen strains. See, for example Jones et al., Science 266: 789 (1994)(cloning of the tomato Cf-9 gene for resistance to Cladosporium fulvum);Martin et al., Science 262:1432 (1993) (tomato Pto gene for resistanceto Pseudomonas syringae pv. tomato encodes a protein kinase); Mindrinoset al., Cell 78:1089 (1994) (Arabidopsis RPS2 gene for resistance toPseudomonas syringae), McDowell & Woffenden, (2003) Trends Biotechnol.21:178-83 and Toyoda et al., (2002) Transgenic Res. 11:567-82. A plantresistant to a disease is one that is more resistant to a pathogen ascompared to the wild type plant.

(B) A Bacillus thuringiensis (Bt) protein, a derivative thereof or asynthetic polypeptide modeled thereon. See, for example, Geiser et al.,Gene 48:109 (1986), who disclose the cloning and nucleotide sequence ofa Bt delta-endotoxin gene. Moreover, DNA molecules encodingdelta-endotoxin genes can be purchased from American Type CultureCollection (Rockville, Md.), for example, under ATCC Accession Nos.40098, 67136, 31995 and 31998. Other non-limiting examples of Bacillusthuringiensis transgenes being genetically engineered are given in thefollowing patents and patent applications and hereby are incorporated byreference for this purpose: U.S. Pat. Nos. 5,188,960; 5,689,052;5,880,275; 5,986,177; 7,105,332; 7,208,474; WO 91/14778; WO 99/31248; WO01/12731; WO 99/24581; WO 97/40162; US2002/0151709; US2003/0177528;US2005/0138685; US/0070245427; US2007/0245428; US2006/0241042;US2008/0020966; US2008/0020968; US2008/0020967; US2008/0172762;US2008/0172762; and US2009/0005306.

(C) An insect-specific hormone or pheromone such as an ecdysteroid orjuvenile hormone, a variant thereof, a mimetic based thereon, or anantagonist or agonist thereof. See, for example, the disclosure byHammock et al., Nature 344:458 (1990), of baculovirus expression ofcloned juvenile hormone esterase, an inactivator of juvenile hormone.

(D) An insect-specific peptide which, upon expression, disrupts thephysiology of the affected pest. For example, see the disclosures ofRegan, J. Biol. Chem. 269:9 (1994) (expression cloning yields DNA codingfor insect diuretic hormone receptor); Pratt et al., Biochem. Biophys.Res. Comm. 163:1243 (1989) (an allostatin is identified in Diplopterapuntata); Chattopadhyay et al. (2004) Critical Reviews in Microbiology30(1): 33-54 2004; Zjawiony (2004) J Nat Prod 67(2): 300-310; Carlini &Grossi-de-Sa (2002) Toxicon, 40(11): 1515-1539; Ussuf et al. (2001) CurrSci. 80(7): 847-853; and Vasconcelos & Oliveira (2004) Toxicon44(4):385-403. See also U.S. Pat. No. 5,266,317 to Tomalski et al., whodisclose genes encoding insect-specific toxins.

(E) An enzyme responsible for a hyperaccumulation of a monoterpene, asesquiterpene, a steroid, hydroxamic acid, a phenylpropanoid derivativeor another non-protein molecule with insecticidal activity.

(F) An enzyme involved in the modification, including thepost-translational modification, of a biologically active molecule; forexample, a glycolytic enzyme, a proteolytic enzyme, a lipolytic enzyme,a nuclease, a cyclase, a transaminase, an esterase, a hydrolase, aphosphatase, a kinase, a phosphorylase, a polymerase, an elastase, achitinase and a glucanase, whether natural or synthetic. See WO93/02197, which discloses the nucleotide sequence of a callase gene. DNAmolecules which contain chitinase-encoding sequences can be obtained,for example, from the ATCC under Accession Nos. 39637 and 67152. Seealso Kramer et al., Insect Biochem. Molec. Biol. 23:691 (1993), whoteach the nucleotide sequence of a cDNA encoding tobacco hookwormchitinase, and Kawalleck et al., Plant Mol. Biol. 21:673 (1993), whoprovide the nucleotide sequence of the parsley ubi4-2 polyubiquitingene, and U.S. Pat. Nos. 6,563,020; 7,145,060 and 7,087,810.

(G) A molecule that stimulates signal transduction. For example, see thedisclosure by Botella et al., Plant Mol. Biol. 24:757 (1994), ofnucleotide sequences for mung bean calmodulin cDNA clones, and Griess etal., Plant Physiol. 104:1467 (1994), who provide the nucleotide sequenceof a maize calmodulin cDNA clone.

(H) A hydrophobic moment peptide. See WO 95/16776 and U.S. Pat. No.5,580,852 disclosure of peptide derivatives of tachyplesin which inhibitfungal plant pathogens, and WO 95/18855 and U.S. Pat. No. 5,607,914which teach synthetic antimicrobial peptides that confer diseaseresistance.

(I) A membrane permease, a channel former, or a channel blocker. Forexample, see the disclosure by Jaynes et al., Plant Sci. 89:43 (1993),of heterologous expression of a cecropin-beta lytic peptide analog torender transgenic tobacco plants resistant to Pseudomonas solanacearum.

(J) A viral-invasive protein or a complex toxin derived therefrom. Forexample, the accumulation of viral coat proteins in transformed plantcells imparts resistance to viral infection and/or disease developmenteffected by the virus from which the coat protein gene is derived, aswell as by related viruses. See Beachy et al., Ann. Rev. Phytopathol.28:451 (1990). Coat protein-mediated resistance has been conferred upontransformed plants against alfalfa mosaic virus, cucumber mosaic virus,tobacco streak virus, potato virus X, potato virus Y, tobacco etchvirus, tobacco rattle virus and tobacco mosaic virus.

(K) An insect-specific antibody or an immunotoxin derived therefrom.Thus, an antibody targeted to a critical metabolic function in theinsect gut would inactivate an affected enzyme, killing the insect. Cf.Taylor et al., Abstract #497, Seventh Intl Symposium on MolecularPlant-Microbe Interactions (Edinburgh, Scotland, 1994) (enzymaticinactivation in transgenic tobacco via production of single-chainantibody fragments).

(L) A virus-specific antibody. See, for example, Tavladoraki et al.,Nature 366:469 (1993), who show that transgenic plants expressingrecombinant antibody genes are protected from virus attack.

(M) A developmental-arrestive protein produced in nature by a pathogenor a parasite. Thus, fungal endo alpha-1,4-D-polygalacturonasesfacilitate fungal colonization and plant nutrient release bysolubilizing plant cell wall homo-alpha-1,4-D-galacturonase. See Lamb etal., Bio/Technology 10:1436 (1992). The cloning and characterization ofa gene which encodes a bean endopolygalacturonase-inhibiting protein isdescribed by Toubart et al., Plant J. 2:367 (1992).

(N) A developmental-arrestive protein produced in nature by a plant. Forexample, Logemann et al., Bio/Technology 10:305 (1992), have shown thattransgenic plants expressing the barley ribosome-inactivating gene havean increased resistance to fungal disease.

(O) Genes involved in the systemic acquired resistance (SAR) Responseand/or the pathogenesis related genes. Briggs Current Biology, 5:128-131(1995), Pieterse & Van Loon (2004) Curr. Opin. Plant Bio. 7:456-64; andSomssich (2003) Cell 113:815-6.

(P) Antifungal genes (Cornelissen and Melchers, Plant Physiol.101:709-712, (1993); Parijs et al., Planta 183:258-264, (1991); Bushnellet al., Can. J. Plant Path. 20:137-149 (1998). Also see US2002/0166141;US2007/0274972; US2007/0192899; US20080022426; and U.S. Pat. Nos.6,891,085; 7,306,946; and 7,598,346.

(Q) Detoxification genes, such as for fumonisin, beauvericin,moniliformin and zearalenone and their structurally related derivatives.For example, see U.S. Pat. Nos. 5,716,820; 5,792,931; 5,798,255;5,846,812; 6,083,736; 6,538,177; 6,388,171 and 6,812,380.

(R) Cystatin and cysteine proteinase inhibitors. See U.S. Pat. No.7,205,453.

(S) Defensin genes. See WO 03/000863 and U.S. Pat. Nos. 6,911,577;6,855,865; 6,777,592 and 7,238,781.

(T) Genes conferring resistance to nematodes. See e.g. WO 96/30517; WO93/19181, WO 03/033651; and Urwin et al., Planta 204:472-479 (1998);Williamson (1999) Curr Opin Plant Bio. 2:327-31; and U.S. Pat. Nos.6,284,948 and 7,301,069.

(U) Genes that confer resistance to Phytophthora Root Rot, such as theRps 1, Rps 1-a, Rps 1-b, Rps 1-c, Rps 1-d, Rps 1-e, Rps 1-k, Rps 2, Rps3-a, Rps 3-b, Rps 3-c, Rps 4, Rps 5, Rps 6, Rps 7 and other Rps genes.See, for example, Shoemaker et al, Phytophthora Root Rot Resistance GeneMapping in Soybean, Plant Genome IV Conference, San Diego, Calif.(1995).

(V) Genes that confer resistance to Brown Stem Rot, such as described inU.S. Pat. No. 5,689,035 and incorporated by reference for this purpose.

2. Transgenes That Confer Resistance To A Herbicide, For Example:

(A) A herbicide that inhibits the growing point or meristem, such as animidazolinone or a sulfonylurea. Exemplary genes in this category codefor mutant ALS and AHAS enzyme as described, for example, by Lee et al.,EMBO J. 7:1241 (1988); and Miki et al., Theor. Appl. Genet. 80:449(1990), respectively. See also, U.S. Pat. Nos. 5,605,011; 5,013,659;5,141,870; 5,767,361; 5,731,180; 5,304,732; 4,761,373; 5,331,107;5,928,937; and 5,378,824; US2007/0214515; and WO 96/33270.

(B) Glyphosate (resistance imparted by mutant5-enolpyruvl-3-phosphikimate synthase (EPSP) and aroA genes,respectively) and other phosphono compounds such as glufosinate(phosphinothricin acetyl transferase (PAT) and Streptomyceshygroscopicus phosphinothricin acetyl transferase (bar) genes), andpyridinoxy or phenoxy proprionic acids and cyclohexones (ACCaseinhibitor-encoding genes). See, for example, U.S. Pat. No. 4,940,835 toShah et al., which discloses the nucleotide sequence of a form of EPSPSwhich can confer glyphosate resistance. U.S. Pat. No. 5,627,061 to Barryet al. also describes genes encoding EPSPS enzymes. See also U.S. Pat.Nos. 6,566,587; 6,338,961; 6,248,876; 6,040,497; 5,804,425; 5,633,435;5,145,783; 4,971,908; 5,312,910; 5,188,642; 4,940,835; 5,866,775;6,225,114; 6,130,366; 5,310,667; 4,535,060; 4,769,061; 5,633,448;5,510,471; Re. 36,449; RE 37,287 E; and 5,491,288; and internationalpublications EP1173580; WO 01/66704; EP1173581 and EP1173582, which areincorporated herein by reference for this purpose. Glyphosate resistanceis also imparted to plants that express a gene that encodes a glyphosateoxido-reductase enzyme as described more fully in U.S. Pat. Nos.5,776,760 and 5,463,175, which are incorporated herein by reference forthis purpose. In addition glyphosate resistance can be imparted toplants by the over expression of genes encoding glyphosateN-acetyltransferase. See, for example, U.S. Application Serial Nos.US2004/0082770; US2005/0246798; and US2008/0234130. A DNA moleculeencoding a mutant aroA gene can be obtained under ATCC accession No.39256, and the nucleotide sequence of the mutant gene is disclosed inU.S. Pat. No. 4,769,061 to Comai. European Patent Application No. 0 333033 to Kumada et al. and U.S. Pat. No. 4,975,374 to Goodman et al.disclose nucleotide sequences of glutamine synthetase genes which conferresistance to herbicides such as L-phosphinothricin. The nucleotidesequence of a phosphinothricin-acetyl-transferase gene is provided inEuropean Patent No. 0 242 246 and 0 242 236 to Leemans et al. De Greefet al., Bio/Technology 7:61 (1989), describe the production oftransgenic plants that express chimeric bar genes coding forphosphinothricin acetyl transferase activity. See also, U.S. Pat. Nos.5,969,213; 5,489,520; 5,550,318; 5,874,265; 5,919,675; 5,561,236;5,648,477; 5,646,024; 6,177,616; and 5,879,903, which are incorporatedherein by reference for this purpose. Exemplary genes conferringresistance to phenoxy proprionic acids and cyclohexones, such assethoxydim and haloxyfop, are the Acc1-S1, Acc1-S2 and Acc1-S3 genesdescribed by Marshall et al., Theor. Appl. Genet. 83:435 (1992).

(C) A herbicide that inhibits photosynthesis, such as a triazine (psbAand gs+genes) and a benzonitrile (nitrilase gene). Przibilla et al.,Plant Cell 3:169 (1991), describe the transformation of Chlamydomonaswith plasmids encoding mutant psbA genes. Nucleotide sequences fornitrilase genes are disclosed in U.S. Pat. No. 4,810,648 to Stalker, andDNA molecules containing these genes are available under ATCC AccessionNos. 53435, 67441 and 67442. Cloning and expression of DNA coding for aglutathione S-transferase is described by Hayes et al., Biochem. J.285:173 (1992).

(D) Acetohydroxy acid synthase, which has been found to make plants thatexpress this enzyme resistant to multiple types of herbicides, has beenintroduced into a variety of plants (see, e.g., Hattori et al. (1995)Mol Gen Genet. 246:419). Other genes that confer resistance toherbicides include: a gene encoding a chimeric protein of rat cytochromeP4507A1 and yeast NADPH-cytochrome P450 oxidoreductase (Shiota et al.(1994) Plant Physiol 106:17), genes for glutathione reductase andsuperoxide dismutase (Aono et al. (1995) Plant Cell Physiol 36:1687, andgenes for various phosphotransferases (Datta et al. (1992) Plant MolBiol 20:619).

(E) Protoporphyrinogen oxidase (protox) is necessary for the productionof chlorophyll, which is necessary for all plant survival. The protoxenzyme serves as the target for a variety of herbicidal compounds. Theseherbicides also inhibit growth of all the different species of plantspresent, causing their total destruction. The development of plantscontaining altered protox activity which are resistant to theseherbicides are described in U.S. Pat. Nos. 6,288,306; 6,282,837; and5,767,373; and WO 01/12825.

3. Transgenes That Confer Or Contribute To an Altered GrainCharacteristic, Such As:

(A) Altered fatty acids, for example, by

-   -   (1) Down-regulation of stearoyl-ACP desaturase to increase        stearic acid content of the plant. See Knultzon et al., Proc.        Natl. Acad. Sci. USA 89:2624 (1992) and WO 99/64579 (Genes for        Desaturases to Alter Lipid Profiles in Corn),    -   (2) Elevating oleic acid via FAD-2 gene modification and/or        decreasing linolenic acid via FAD-3 gene modification (see U.S.        Pat. Nos. 6,063,947; 6,323,392; 6,372,965; and WO 93/11245),    -   (3) Altering conjugated linolenic or linoleic acid content, such        as in WO 01/12800,    -   (4) Altering LEC1, AGP, Dek1, Superan, mi1 ps, various Ipa genes        such as Ipa1, Ipa3, hpt or hggt. For example, see WO 02/42424;        WO 98/22604; WO 03/011015; U.S. Pat. No. 6,423,886; U.S. Pat.        No. 6,197,561; U.S. Pat. No. 6,825,397; US2003/0079247;        US2003/0204870; WO 02/057439; WO 03/011015; and        Rivera-Madrid, R. et al. Proc. Natl. Acad. Sci. 92:5620-5624        (1995).

B) Altered phosphorus content, for example, by the

-   -   (1) Introduction of a phytase-encoding gene would enhance        breakdown of phytate, adding more free phosphate to the        transformed plant. For example, see Van Hartingsveldt et al.,        Gene 127:87 (1993), for a disclosure of the nucleotide sequence        of an Aspergillus niger phytase gene.    -   (2) Modulating a gene that reduces phytate content. In maize,        this, for example, could be accomplished, by cloning and then        re-introducing DNA associated with one or more of the alleles,        such as the LPA alleles, identified in maize mutants        characterized by low levels of phytic acid, such as in WO        05/113778 and/or by altering inositol kinase activity as in WO        02/059324, U.S. Pat. No. 7,067,720, WO 03/027243,        US2003/0079247, WO 99/05298, U.S. Pat. No. 6,197,561, U.S. Pat.        No. 6,291,224, U.S. Pat. No. 6,391,348, WO 98/45448, WO        99/55882, WO 01/04147.

(C) Altered carbohydrates, for example, by altering a gene for an enzymethat affects the branching pattern of starch or, a gene alteringthioredoxin such as NTR and/or TRX (see U.S. Pat. No. 6,531,648 which isincorporated by reference for this purpose) and/or a gamma zein knockout or mutant such as cs27 or TUSC27 or en27 (See U.S. Pat. No.6,858,778; US2005/0160488; and US2005/0204418; which are incorporated byreference for this purpose). See Shiroza et al., J. Bacteriol. 170:810(1988) (nucleotide sequence of Streptococcus mutans fructosyltransferasegene), Steinmetz et al., Mol. Gen. Genet. 200:220 (1985) (nucleotidesequence of Bacillus subtilis levansucrase gene), Pen et al.,Bio/Technology 10:292 (1992) (production of transgenic plants thatexpress Bacillus licheniformis alpha-amylase), Elliot et al., Plant Mol.Biol. 21:515 (1993) (nucleotide sequences of tomato invertase genes),Søgaard et al., J. Biol. Chem. 268:22480 (1993) (site-directedmutagenesis of barley alpha-amylase gene), and Fisher et al., PlantPhysiol. 102:1045 (1993) (maize endosperm starch branching enzyme II),WO 99/10498 (improved digestibility and/or starch extraction throughmodification of UDP-D-xylose 4-epimerase, Fragile 1 and 2, Reft HCHL,C4H), U.S. Pat. No. 6,232,529 (method of producing high oil seed bymodification of starch levels (AGP)). The fatty acid modification genesmentioned herein may also be used to affect starch content and/orcomposition through the interrelationship of the starch and oilpathways.

(D) Altered antioxidant content or composition, such as alteration oftocopherol or tocotrienols. For example, see U.S. Pat. No. 6,787,683;U.S. Pat. No. 7,154,029; and WO 00/68393 involving the manipulation ofantioxidant levels, and WO 03/082899 through alteration of ahomogentisate geranyl geranyl transferase (hggt).

(E) Altered essential seed amino acids. For example, see U.S. Pat. No.6,127,600 (method of increasing accumulation of essential amino acids inseeds), U.S. Pat. No. 6,080,913 (binary methods of increasingaccumulation of essential amino acids in seeds), U.S. Pat. No. 5,990,389(high lysine), WO 99/40209 (alteration of amino acid compositions inseeds), WO 99/29882 (methods for altering amino acid content ofproteins), U.S. Pat. No. 5,850,016 (alteration of amino acidcompositions in seeds), WO 98/20133 (proteins with enhanced levels ofessential amino acids), U.S. Pat. No. 5,885,802 (high methionine), U.S.Pat. No. 5,885,801 (high threonine), U.S. Pat. No. 6,664,445 (plantamino acid biosynthetic enzymes), U.S. Pat. No. 6,459,019 (increasedlysine and threonine), U.S. Pat. No. 6,441,274 (plant tryptophansynthase beta subunit), U.S. Pat. No. 6,346,403 (methionine metabolicenzymes), U.S. Pat. No. 5,939,599 (high sulfur), U.S. Pat. No. 5,912,414(increased methionine), WO 98/56935 (plant amino acid biosyntheticenzymes), WO 98/45458 (engineered seed protein having higher percentageof essential amino acids), WO 98/42831 (increased lysine), U.S. Pat. No.5,633,436 (increasing sulfur amino acid content), U.S. Pat. No.5,559,223 (synthetic storage proteins with defined structure containingprogrammable levels of essential amino acids for improvement of thenutritional value of plants), WO 96/01905 (increased threonine), WO95/15392 (increased lysine), U.S. Pat. No. 6,930,225, U.S. Pat. No.7,179,955, US2004/0068767, U.S. Pat. No. 6,803,498, WO 01/79516.

4. Genes that Control Male-sterility

There are several methods of conferring genetic male sterilityavailable, such as multiple mutant genes at separate locations withinthe genome that confer male sterility, as disclosed in U.S. Pat. Nos.4,654,465 and 4,727,219 to Brar et al. and chromosomal translocations asdescribed by Patterson in U.S. Pat. Nos. 3,861,709 and 3,710,511. Inaddition to these methods, Albertsen et al., U.S. Pat. No. 5,432,068,describe a system of nuclear male sterility which includes: identifyinga gene which is critical to male fertility; silencing this native genewhich is critical to male fertility; removing the native promoter fromthe essential male fertility gene and replacing it with an induciblepromoter; inserting this genetically engineered gene back into theplant; and thus creating a plant that is male sterile because theinducible promoter is not “on” resulting in the male fertility gene notbeing transcribed. Fertility is restored by inducing, or turning “on”,the promoter, which in turn allows the gene that confers male fertilityto be transcribed. Male sterile soybean lines and characterization arediscussed in Palmer (2000) Crop Sci 40:78-83, and Jin et al. (1997) SexPlant Reprod 10:13-21.

(A) Introduction of a deacetylase gene under the control of atapetum-specific promoter and with the application of the chemicalN—Ac—PPT (WO 01/29237).

(B) Introduction of various stamen-specific promoters (WO 92/13956, WO92/13957).

(C) Introduction of the barnase and the barstar gene (Paul et al. PlantMol. Biol. 19:611-622, 1992).

For additional examples of nuclear male and female sterility systems andgenes, see also, U.S. Pat. Nos. 5,859,341; 6,297,426; 5,478,369;5,824,524; 5,850,014; and 6,265,640; all of which are herebyincorporated by reference.

5. Polynucleotides that create a site for site specific DNA integration.This includes the introduction of at least one FRT site that may be usedin the FLP/FRT system and/or a Lox site that may be used in the Cre/Loxsystem. For example, see Lyznik et al., Site-Specific Recombination forGenetic Engineering in Plants, Plant Cell Rep (2003) 21:925-932 and WO99/25821, which are hereby incorporated by reference. Other systems thatmay be used include the Gin recombinase of phage Mu (Maeser et al.(1991) Mol Gen Genet. 230:170-176); the Pin recombinase of E. coli(Enomoto et al. (1983) J Bacteriol 156:663-668); and the R/RS system ofthe pSRi plasmid (Araki et al. (1992) J Mol Biol 182:191-203).6. Genes that affect abiotic stress resistance (including but notlimited to flowering, ear and seed development, enhancement of nitrogenutilization efficiency, altered nitrogen responsiveness, droughtresistance or tolerance, cold resistance or tolerance, and saltresistance or tolerance) and increased yield under stress. For example,see: WO 00/73475 where water use efficiency is altered throughalteration of malate; U.S. Pat. Nos. 5,892,009, 5,965,705, 5,929,305,5,891,859, 6,417,428, 6,664,446, 6,706,866, 6,717,034, 6,801,104, WO00/060089, WO 01/026459, WO 00/1035725, WO 01/034726, WO 01/035727, WO00/1036444, WO 01/036597, WO 01/036598, WO 00/2015675, WO 02/017430, WO02/077185, WO 02/079403, WO 03/013227, WO 03/013228, WO 03/014327, WO04/031349, WO 04/076638, WO 98/09521, and WO 99/38977 describing genes,including CBF genes and transcription factors effective in mitigatingthe negative effects of freezing, high salinity, and drought on plants,as well as conferring other positive effects on plant phenotype;US2004/0148654 and WO 01/36596 where abscisic acid is altered in plantsresulting in improved plant phenotype such as increased yield and/orincreased tolerance to abiotic stress; WO 00/006341, WO 04/090143, U.S.Pat. No. 7,531,723 and U.S. Pat. No. 6,992,237 where cytokininexpression is modified resulting in plants with increased stresstolerance, such as drought tolerance, and/or increased yield. Also seeWO 02/02776, WO 03/052063, JP2002281975, U.S. Pat. No. 6,084,153, WO01/64898, U.S. Pat. No. 6,177,275, and U.S. Pat. No. 6,107,547(enhancement of nitrogen utilization and altered nitrogenresponsiveness). For ethylene alteration, see US2004/0128719,US2003/0166197, and WO 00/32761. For plant transcription factors ortranscriptional regulators of abiotic stress, see e.g. US2004/0098764 orUS2004/0078852.

Other genes and transcription factors that affect plant growth andagronomic traits such as yield, flowering, plant growth and/or plantstructure, can be introduced or introgressed into plants, see e.g., WO97/49811 (LHY), WO 98/56918 (ESD4), WO 97/10339 and U.S. Pat. No.6,573,430 (TFL), U.S. Pat. No. 6,713,663 (FT), WO 96/14414 (CON), WO96/38560, WO 01/21822 (VRN1), WO 00/44918 (VRN2), WO 99/49064 (GI), WO00/46358 (FR1), WO 97/29123, U.S. Pat. No. 6,794,560, U.S. Pat. No.6,307,126 (GAI), WO 99/09174 (D8 and Rht), and WO 04/076638 and WO04/031349 (transcription factors).

Development of Soybean Sublines

Sublines of XR28L10 may also be developed. Although XR28L10 containssubstantially fixed genetics and is phenotypically uniform with nooff-types expected, there still remains a small proportion ofsegregating loci either within individuals or within the population as awhole. Sublining provides the ability to select for these loci, whichhave no apparent morphological or phenotypic effect on the plantcharacteristics, but may have an affect on overall yield. For example,the methods described in U.S. Pat. No. 5,437,697 and US2005/0071901 maybe utilized by a breeder of ordinary skill in the art to identifygenetic loci that are associated with yield potential to further purifythe variety in order to increase its yield (both of which are hereinincorporated by reference). Based on these teachings, a breeder ofordinary skill in the art may fix agronomically important loci by makingthem homozygous in order to optimize the performance of the variety. Nocrosses to a different variety are made, and so a new genetic variety isnot created and the overall genetic composition of the variety remainsessentially the same. The development of soybean sublines and the use ofaccelerated yield technology is a plant breeding technique.

Soybean varieties such as XR28L10 are typically developed for use inseed and grain production. However, soybean varieties such as XR28L10also provide a source of breeding material that may be used to developnew soybean varieties. Plant breeding techniques known in the art andused in a soybean plant breeding program include, but are not limitedto, recurrent selection, mass selection, bulk selection, backcrossing,pedigree breeding, open pollination breeding, restriction fragmentlength polymorphism enhanced selection, genetic marker enhancedselection, making double haploids, and transformation. Oftencombinations of these techniques are used. The development of soybeanvarieties in a plant breeding program requires, in general, thedevelopment and evaluation of homozygous varieties. There are manyanalytical methods available to evaluate a new variety. The oldest andmost traditional method of analysis is the observation of phenotypictraits but genotypic analysis may also be used.

Methods for producing a soybean plant by crossing a first parent soybeanplant with a second parent soybean plant wherein the first and/or secondparent soybean plant is variety XR28L10 are provided. The other parentmay be any soybean plant, such as a soybean plant that is part of asynthetic or natural population. Any such methods using soybean varietyXR28L10 include but are not limited to: selfing, sibbing, backcrossing,mass selection, pedigree breeding, bulk selection, hybrid production,crossing to populations, and the like. These methods are well known inthe art and some of the more commonly used breeding methods aredescribed below. Descriptions of breeding methods can be found in one ofseveral reference books (e.g., Allard, Principles of Plant Breeding,1960; Simmonds, Principles of Crop Improvement, 1979; Sneep et al.,1979; Fehr, “Breeding Methods for Cultivar Development”, Chapter 7,Soybean Improvement, Production and Uses, 2^(nd) ed., Wilcox editor,1987).

Pedigree breeding starts with the crossing of two genotypes, such asXR28L10 and another soybean variety having one or more desirablecharacteristics that is lacking or which complements XR28L10. If the twooriginal parents do not provide all the desired characteristics, othersources can be included in the breeding population. In the pedigreemethod, superior plants are selfed and selected in successive filialgenerations. In the succeeding filial generations, the heterozygousallele condition gives way to the homozygous allele condition as aresult of inbreeding. Typically in the pedigree method of breeding, fiveor more successive filial generations of selfing and selection ispracticed: F1→F2; F2→F3; F3→F4; F4→F5, etc. After a sufficient amount ofinbreeding, successive filial generations will serve to increase seed ofthe developed variety. Typically, the developed variety compriseshomozygous alleles at about 95% or more of its loci.

In addition to being used to create backcross conversion populations,backcrossing can also be used in combination with pedigree breeding. Asdiscussed previously, backcrossing can be used to transfer one or morespecifically desirable traits from one variety (the donor parent) to adeveloped variety (the recurrent parent), which has overall goodagronomic characteristics yet lacks that desirable trait or traits.However, the same procedure can be used to move the progeny toward thegenotype of the recurrent parent but at the same time retain manycomponents of the non-recurrent parent by stopping the backcrossing atan early stage and proceeding with selfing and selection. For example, asoybean variety may be crossed with another variety to produce a firstgeneration progeny plant. The first generation progeny plant may then bebackcrossed to one of its parent varieties to create a BC1F1. Progenyare selfed and selected so that the newly developed variety has many ofthe attributes of the recurrent parent and yet several of the desiredattributes of the donor parent. This approach leverages the value andstrengths of the recurrent parent for use in new soybean varieties.

Therefore, in some examples a method of making a backcross conversion ofsoybean variety XR28L10, comprising the steps of crossing a plant ofsoybean variety XR28L10 with a donor plant possessing a desired trait,selecting an F1 progeny plant containing the desired trait, andbackcrossing the selected F1 progeny plant to a plant of soybean varietyXR28L10 are provided. This method may further comprise the step ofobtaining a molecular marker profile of soybean variety XR28L10 andusing the molecular marker profile to select for a progeny plant withthe desired trait and the molecular marker profile of XR28L10. In oneexample the desired trait is a mutant gene or transgene present in thedonor parent.

Recurrent selection is a method used in a plant breeding program toimprove a population of plants. XR28L10 is suitable for use in arecurrent selection program. The method entails individual plants crosspollinating with each other to form progeny. The progeny are grown andthe superior progeny selected by any number of selection methods, whichinclude individual plant, half-sib progeny, full-sib progeny and selfedprogeny. The selected progeny are cross pollinated with each other toform progeny for another population. This population is planted andagain superior plants are selected to cross pollinate with each other.Recurrent selection is a cyclical process and therefore can be repeatedas many times as desired. The objective of recurrent selection is toimprove the traits of a population. The improved population can then beused as a source of breeding material to obtain new varieties forcommercial or breeding use, including the production of a syntheticcultivar. A synthetic cultivar is the resultant progeny formed by theintercrossing of several selected varieties.

Mass selection is a useful technique when used in conjunction withmolecular marker enhanced selection. In mass selection seeds fromindividuals are selected based on phenotype or genotype. These selectedseeds are then bulked and used to grow the next generation. Bulkselection requires growing a population of plants in a bulk plot,allowing the plants to self-pollinate, harvesting the seed in bulk andthen using a sample of the seed harvested in bulk to plant the nextgeneration. Also, instead of self pollination, directed pollinationcould be used as part of the breeding program.

Mutation breeding is another method of introducing new traits intosoybean variety XR28L10. Mutations that occur spontaneously or areartificially induced can be useful sources of variability for a plantbreeder. The goal of artificial mutagenesis is to increase the rate ofmutation for a desired characteristic. Mutation rates can be increasedby many different means including temperature, long-term seed storage,tissue culture conditions, radiation; such as X-rays, Gamma rays (e.g.,cobalt 60 or cesium 137), neutrons, (product of nuclear fission byuranium 235 in an atomic reactor), beta radiation (emitted fromradioisotopes such as phosphorus 32 or carbon 14), ultraviolet radiation(preferably from 2500 to 2900 nm), or chemical mutagens such as baseanalogues (5-bromo-uracil), related compounds (8-ethoxy caffeine),antibiotics (streptonigrin), alkylating agents (sulfur mustards,nitrogen mustards, epoxides, ethylenamines, sulfates, sulfonates,sulfones, lactones), azide, hydroxylamine, nitrous acid, or acridines.Once a desired trait is observed through mutagenesis, the trait may thenbe incorporated into existing germplasm by traditional breedingtechniques. Details of mutation breeding can be found in “Principles ofCultivar Development” Fehr, 1993 Macmillan Publishing Company. Inaddition, mutations created in other soybean plants may be used toproduce a backcross conversion of XR28L10 that comprises such mutation.

Molecular markers, which includes markers identified through the use oftechniques such as isozyme electrophoresis, restriction fragment lengthpolymorphisms (RFLPs), randomly amplified polymorphic DNAs (RAPDs),arbitrarily primed polymerase chain reaction (AP-PCR), DNA amplificationfingerprinting (DAF), sequence characterized amplified regions (SCARs),amplified fragment length polymorphisms (AFLPs), simple sequence repeats(SSRs) and single nucleotide polymorphisms (SNPs), may be used in plantbreeding methods utilizing XR28L10.

Isozyme electrophoresis and RFLPs have been widely used to determinegenetic composition. Shoemaker and Olsen, ((1993) Molecular Linkage Mapof Soybean (Glycine max L. Merr.). p. 6.131-6.138. In S. J. O'Brien(ed.) Genetic Maps: Locus Maps of Complex Genomes. Cold Spring HarborLaboratory Press. Cold Spring Harbor, N.Y.), developed a moleculargenetic linkage map that consisted of 25 linkage groups with about 365RFLP, 11 RAPD (random amplified polymorphic DNA), three classicalmarkers, and four isozyme loci. See also, Shoemaker R. C. 1994 RFLP Mapof Soybean. P. 299-309 In R. L. Phillips and I. K. Vasil (ed.) DNA-basedmarkers in plants. Kluwer Academic Press Dordrecht, the Netherlands.

SSR technology is an efficient and practical marker technology; moremarker loci can be routinely used and more alleles per marker locus canbe found using SSRs in comparison to RFLPs. For example Diwan andCregan, described a highly polymorphic microsatellite loci in soybeanwith as many as 26 alleles. (Diwan, N., and P. B. Cregan 1997 Automatedsizing of fluorescent-labeled simple sequence repeat (SSR) markers toassay genetic variation in Soybean Theor. Appl. Genet. 95:220-225).Single nucleotide polymorphisms (SNPs) may also be used to identify theunique genetic composition of the XR28L10 and progeny varietiesretaining or derived from that unique genetic composition. Variousmolecular marker techniques may be used in combination to enhanceoverall resolution.

Soybean DNA molecular marker linkage maps have been rapidly constructedand widely implemented in genetic studies. One such study is describedin Cregan et. al, “An Integrated Genetic Linkage Map of the SoybeanGenome” Crop Science 39:1464-1490 (1999). Sequences and PCR conditionsof SSR Loci in Soybean as well as the most current genetic map may befound in Soybase on the world wide web.

One use of molecular markers is quantitative trait loci (QTL) mapping.QTL mapping is the use of markers, which are known to be closely linkedto alleles that have measurable effects on a quantitative trait.Selection in the breeding process is based upon the accumulation ofmarkers linked to the positive effecting alleles and/or the eliminationof the markers linked to the negative effecting alleles from the plantgenome.

Molecular markers can also be used during the breeding process for theselection of qualitative traits. For example, markers closely linked toalleles or markers containing sequences within the actual alleles ofinterest can be used to select plants that contain the alleles ofinterest during a backcrossing breeding program. The markers can also beused to select for the genome of the recurrent parent and against thegenome of the donor parent. Using this procedure can minimize the amountof genome from the donor parent that remains in the selected plants. Itcan also be used to reduce the number of crosses back to the recurrentparent needed in a backcrossing program. The use of molecular markers inthe selection process is often called genetic marker enhanced selection.

Production of Double Haploids

The production of double haploids can also be used for the developmentof plants with a homozygous phenotype in the breeding program. Forexample, a soybean plant for which XR28L10 is a parent can be used toproduce double haploid plants. Double haploids are produced by thedoubling of a set of chromosomes (1 N) from a heterozygous plant toproduce a completely homozygous individual. For example, see Wan et al.,“Efficient Production of Doubled Haploid Plants Through ColchicineTreatment of Anther-Derived Maize Callus”, Theoretical and AppliedGenetics, 77:889-892, 1989 and US2003/0005479. This can be advantageousbecause the process omits the generations of selfing needed to obtain ahomozygous plant from a heterozygous source.

Methods for obtaining haploid plants are disclosed in Kobayashi, M. etal., J Heredity 71:9-14, 1980, Pollacsek, M., Agronomie (Paris)12:247-251, 1992; Cho-Un-Haing et al., J Plant Biol., 1996, 39:185-188;Verdoodt, L., et al., 1998, 96:294-300; Genetic Manipulation in PlantBreeding, Proceedings International Symposium Organized by EUCARPIA,Sep. 8-13, 1985, Berlin, Germany; Chalyk et al., 1994, Maize Genet Coop.Newsletter 68:47. Double haploid technology in soybean is discussed inCroser et al. (2006) Crit. Rev Plant Sci 25:139-157; and Rodrigues etal. (2006) Brazilian Arc Biol Tech 49:537-545.

In some examples a process for making a substantially homozygous XR28L10progeny plant by producing or obtaining a seed from the cross of XR28L10and another soybean plant and applying double haploid methods to the F1seed or F1 plant or to any successive filial generation is provided.Based on studies in maize and currently being conducted in soybean, suchmethods would decrease the number of generations required to produce avariety with similar genetics or characteristics to XR28L10. SeeBernardo, R. and Kahler, A. L., Theor. Appl. Genet. 102:986-992, 2001.

In particular, a process of making seed retaining the molecular markerprofile of soybean variety XR28L10 is contemplated, such processcomprising obtaining or producing F1 seed for which soybean varietyXR28L10 is a parent, inducing doubled haploids to create progeny withoutthe occurrence of meiotic segregation, obtaining the molecular markerprofile of soybean variety XR28L10, and selecting progeny that retainthe molecular marker profile of XR28L10.

Methods using seeds, plants, cells, or plant parts of variety XR28L10 intissue culture are provided, as are the cultures, plants, parts, cells,and/or seeds derived therefrom. Tissue culture of various tissues ofsoybeans and regeneration of plants therefrom is well known and widelypublished. For example, see Komatsuda, T. et al., “Genotype X SucroseInteractions for Somatic Embryogenesis in Soybean,” Crop Sci. 31:333-337(1991); Stephens, P. A. et al., “Agronomic Evaluation ofTissue-Culture-Derived Soybean Plants,” Theor. Appl. Genet. (1991)82:633-635; Komatsuda, T. et al., “Maturation and Germination of SomaticEmbryos as Affected by Sucrose and Plant Growth Regulators in SoybeansGlycine gracilis Skvortz and Glycine max (L.) Merr.,” Plant Cell, Tissueand Organ Culture, 28:103-113 (1992); Dhir, S. et al., “Regeneration ofFertile Plants from Protoplasts of Soybean (Glycine max L. Merr.):Genotypic Differences in Culture Response,” Plant Cell Reports (1992)11:285-289; Pandey, P. et al., “Plant Regeneration from Leaf andHypocotyl Explants of Glycine wightii (W. and A.) VERDC. var.longicauda,” Japan J. Breed. 42:1-5 (1992); and Shetty, K., et al.,“Stimulation of In Vitro Shoot Organogenesis in Glycine max (Merrill.)by Allantoin and Amides,” Plant Science 81:(1992) 245-251; as well asU.S. Pat. No. 5,024,944, issued Jun. 18, 1991 to Collins et al. and U.S.Pat. No. 5,008,200, issued Apr. 16, 1991 to Ranch et al., thedisclosures of which are hereby incorporated herein in their entirety byreference. Thus, another aspect is to provide cells which upon growthand differentiation produce soybean plants having the physiological andmorphological characteristics of soybean variety XR28L10.

REFERENCES

-   Aukerman, M. J. et al. (2003) “Regulation of Flowering Time and    Floral Organ Identity by a MicroRNA and Its APETALA2-like Target    Genes” The Plant Cell 15:2730-2741-   Berry et al., Assessing Probability of Ancestry Using Simple    Sequence Repeat Profiles: Applications to Maize Inbred Lines and    Soybean Varieties” Genetics 165:331-342 (2003)-   Boppenmaier, et al., “Comparisons Among Strains of Inbreds for    RFLPs”, Maize Genetics Cooperative Newsletter, 65:1991, p. 90-   Conger, B. V., et al. (1987) “Somatic Embryogenesis From Cultured    Leaf Segments of Zea Mays”, Plant Cell Reports, 6:345-347-   Cregan et al, “An Integrated Genetic Linkage Map of the Soybean    Genome” Crop Science 39:1464-1490 (1999).-   Diwan et al., “Automated sizing of fluorescent-labeled simple    sequence repeat (SSR) markers to assay genetic variation in Soybean”    Theor. Appl. Genet. 95:220-225. (1997).-   Duncan, D. R., et al. (1985) “The Production of Callus Capable of    Plant Regeneration From Immature Embryos of Numerous Zea Mays    Genotypes”, Planta, 165:322-332-   Edallo, et al. (1981) “Chromosomal Variation and Frequency of    Spontaneous Mutation Associated with in Vitro Culture and Plant    Regeneration in Maize”, Maydica, XXVI: 39-56-   Fehr, Walt, Principles of Cultivar Development, pp. 261-286 (1987)-   Green, et al. (1975) “Plant Regeneration From Tissue Cultures of    Maize”, Crop Science, Vol. 15, pp. 417-421-   Green, C. E., et al. (1982) “Plant Regeneration in Tissue Cultures    of Maize” Maize for Biological Research, pp. 367-372-   Hallauer, A. R. et al. (1988) “Corn Breeding” Corn and Corn    Improvement, No. 18, pp. 463-481-   Lee, Michael (1994) “Inbred Lines of Maize and Their Molecular    Markers”, The Maize Handbook, Ch. 65:423-432-   Meghji, M. R., et al. (1984) “Inbreeding Depression, Inbred & Hybrid    Grain Yields, and Other Traits of Maize Genotypes Representing Three    Eras”, Crop Science, Vol. 24, pp. 545-549-   Openshaw, S. J., et al. (1994) “Marker-assisted selection in    backcross breeding”. pp. 41-43. In Proceedings of the Symposium    Analysis of Molecular Marker Data. 5-7 Aug. 1994. Corvallis, Oreg.,    American Society for Horticultural Science/Crop Science Society of    America-   Phillips, et al. (1988) “Cell/Tissue Culture and In Vitro    Manipulation”, Corn & Corn Improvement, 3rd Ed., ASA Publication,    No. 18, pp. 345-387-   Poehlman et al (1995) Breeding Field Crops, 4th Ed., Iowa State    University Press, Ames, Iowa., pp. 132-155 and 321-344-   Rao, K. V., et al., (1986) “Somatic Embryogenesis in Glume Callus    Cultures”, Maize Genetics Cooperative Newsletter, No. 60, pp. 64-65-   Sass, John F. (1977) “Morphology”, Corn & Corn Improvement, ASA    Publication, Madison, Wis. pp. 89-109-   Smith, J. S. C., et al., “The Identification of Female Selfs in    Hybrid Maize: A Comparison Using Electrophoresis and Morphology”,    Seed Science and Technology 14, 1-8-   Songstad, D. D. et al. (1988) “Effect of ACC    (1-aminocyclopropane-1-carboyclic acid), Silver Nitrate &    Norbonadiene on Plant Regeneration From Maize Callus Cultures”,    Plant Cell Reports, 7:262-265-   Tomes, et al. (1985) “The Effect of Parental Genotype on Initiation    of Embryogenic Callus From Elite Maize (Zea Mays L.) Germplasm”,    Theor. Appl. Genet., Vol. 70, p. 505-509-   Troyer, et al. (1985) “Selection for Early Flowering in Corn: 10    Late Synthetics”, Crop Science, Vol. 25, pp. 695-697-   Umbeck, et al. (1983) “Reversion of Male-Sterile T-Cytoplasm Maize    to Male Fertility in Tissue Culture”, Crop Science, Vol. 23, pp.    584-588-   Wan et al., “Efficient Production of Doubled Haploid Plants Through    Colchicine Treatment of Anther-Derived Maize Callus”, Theoretical    and Applied Genetics, 77:889-892, 1989-   Wright, Harold (1980) “Commercial Hybrid Seed Production”,    Hybridization of Crop Plants, Ch. 8:161-176-   Wych, Robert D. (1988) “Production of Hybrid Seed”, Corn and Corn    Improvement, Ch. 9, pp. 565-607    Deposits

Applicant made a deposit of seeds of Soybean Variety XR28L10 with theAmerican Type Culture Collection (ATCC), Manassas, Va. 20110 USA, ATCCDeposit No. PTA-12685. The seeds deposited with the ATCC on Mar. 15,2012 were taken from the seed stock maintained by Pioneer Hi-BredInternational, Inc., 7250 NW 62^(nd) Avenue, Johnston, Iowa 50131 sinceprior to the filing date of this application. Access to this seed stockwill be available during the pendency of the application to theCommissioner of Patents and Trademarks and persons determined by theCommissioner to be entitled thereto upon request. Upon allowance of anyclaims in the application, the Applicant will make the deposit availableto the public pursuant to 37 C.F.R. §1.808. This deposit of SoybeanVariety XR28L10 will be maintained in the ATCC depository, which is apublic depository, for a period of 30 years, or 5 years after the mostrecent request, or for the enforceable life of the patent, whichever islonger, and will be replaced if it becomes nonviable during that period.Additionally, Applicant has or will satisfy all the requirements of 37C.F.R. §§1.801-1.809, including providing an indication of the viabilityof the sample upon deposit. Applicant has no authority to waive anyrestrictions imposed by law on the transfer of biological material orits transportation in commerce. Applicant does not waive anyinfringement of their rights granted under this patent or under thePlant Variety Protection Act (7 USC 2321 et seq.).

TABLE 1 Variety Description Information for XR28L10 Current Variety NameXR28L10 Relative Maturity 28 Herbicide Resistance RR HarvestStandability Field Emergence Hypocotyl Length Phytophthora Gene 1KPhytophthora Race 5 Phytophthora Race 7 Phytophthora Race 25Phytophthora Field Tolerance Brown Stem Rot Iron Chlorosis White MoldTolerance Sudden Death Syndrome Cyst Nematode Race 1 Cyst Nematode Race2 Cyst Nematode Race 3 Cyst Nematode Race 5 Cyst Nematode Race 14 AphidAntibiosis Root-knot Nematode - Southern Root-knot Nematode - PeanutStem Canker Genetic Stem Canker Tolerance Frogeye Leaf Spot Aerial WebBlight Chloride Sensitivity Canopy Width  6 Shattering Plant Habit IndOil/Meal Type LLC; HLC; Seed Protein (% @ 13% H20) 37.2 Seed Oil (% @13% H20) 18.0 Seed Size Score  6 Flower Color W Pubescence Color T HilaColor G Pod Color BR Seed Coat Luster

TABLE 2 VARIETY COMPARISON DATA YIELD bu/a MATABS HGT SPLB 60# count incount Variety1 Variety2 Statistic ABS ABS ABS ABS XR28L10 92M82 Mean156.7 132 31.9 2980 XR28L10 92M82 Mean2 53.2 131.8 36.5 2651 XR28L1092M82 #Locs 17 8 4 5 XR28L10 92M82 #Reps 34 17 7 5 XR28L10 92M82 #Years1 1 1 1 XR28L10 92M82 %Wins 70.6 50 100 100 XR28L10 92M82 Diff 3.5 0.24.6 330 XR28L10 92M82 SE Diff 1.5 0.54 0.47 53.3 XR28L10 92M82 Prob0.034 0.7091 0.0023 0.0035 XR28L10 92M91 Mean1 56.7 132 31.9 2980XR28L10 92M91 Mean2 56.4 132.6 36.3 2889 XR28L10 92M91 #Locs 17 8 4 5XR28L10 92M91 #Reps 34 17 7 5 XR28L10 92M91 #Years 1 1 1 1 XR28L10 92M91%Wins 52.9 12.5 100 80 XR28L10 92M91 Diff 0.3 −0.5 4.4 92 XR28L10 92M91SE Diff 1.13 0.24 1.21 28.3 XR28L10 92M91 Prob 0.8047 0.071 0.03670.0319 XR28L10 92Y61 Mean1 56.7 132 31.9 2980 XR28L10 92Y61 Mean2 53.6129 34.9 2905 XR28L10 92Y61 #Locs 17 8 4 5 XR28L10 92Y61 #Reps 34 17 7 5XR28L10 92Y61 #Years 1 1 1 1 XR28L10 92Y61 %Wins 76.5 87.5 100 80XR28L10 92Y61 Diff 3.1 3 3 75 XR28L10 92Y61 SE Diff 1 0.69 0.71 83.3XR28L10 92Y61 Prob 0.0068 0.0031 0.024 0.4189 XR28L10 92Y70 Mean1 56.7132 31.9 2980 XR28L10 92Y70 Mean2 57.6 129.8 34.5 2738 XR28L10 92Y70#Locs 17 8 4 5 XR28L10 92Y70 #Reps 34 17 7 5 XR28L10 92Y70 #Years 1 1 11 XR28L10 92Y70 %Wins 41.2 100 100 100 XR28L10 92Y70 Diff −0.9 2.3 2.6242 XR28L10 92Y70 SE Diff 1.18 0.4 0.13 48.2 XR28L10 92Y70 Prob 0.4610.0007 0.0002 0.0073 XR28L10 92Y80 Mean1 56.3 131.3 31.9 2980 XR28L1092Y80 Mean2 57.5 131 34.1 2809 XR28L10 92Y80 #Locs 22 10 4 5 XR28L1092Y80 #Reps 39 19 7 5 XR28L10 92Y80 #Years 2 2 1 1 XR28L10 92Y80 %Wins50 40 100 60 XR28L10 92Y80 Diff −1.3 0.3 2.3 171 XR28L10 92Y80 SE Diff1.35 0.6 0.48 88.9 XR28L10 92Y80 Prob 0.3533 0.5917 0.0182 0.127 PROTNOILPCT R160 R180 pct pct pct pct Variety1 Variety2 Statistic ABS ABS ABSABS XR28L10 92M82 Mean1 35.87 17.56 6.26 4.66 XR28L10 92M82 Mean2 33.917.53 9.93 4.46 XR28L10 92M82 #Locs 10 10 8 8 XR28L10 92M82 #Reps 11 1110 10 XR28L10 92M82 #Years 1 1 1 1 XR28L10 92M82 %Wins 100 70 100 12.5XR28L10 92M82 Diff 1.97 0.03 3.67 −0.19 XR28L10 92M82 SE Diff 0.1670.052 0.171 0.055 XR28L10 92M82 Prob 0 0.5902 0 0.0101 XR28L10 92M91Mean1 35.87 17.56 6.26 4.66 XR28L10 92M91 Mean2 32.31 18.91 10.12 4.92XR28L10 92M91 #Locs 10 10 8 8 XR28L10 92M91 #Reps 11 11 10 10 XR28L1092M91 #Years 1 1 1 1 XR28L10 92M91 %Wins 100 0 100 87.5 XR28L10 92M91Diff 3.57 −1.35 3.86 0.26 XR28L10 92M91 SE Diff 0.161 0.118 0.148 0.128XR28L10 92M91 Prob 0 0 0 0.0796 XR28L10 92Y61 Mean1 35.87 17.56 6.264.66 XR28L10 92Y61 Mean2 36.15 17.4 6.69 4.38 XR28L10 92Y61 #Locs 10 108 8 XR28L10 92Y61 #Reps 10 10 10 10 XR28L10 92Y61 #Years 1 1 1 1 XR28L1092Y61 %Wins 20 70 100 37.5 XR28L10 92Y61 Diff −0.27 0.16 0.42 −0.28XR28L10 92Y61 SE Diff 0.138 0.071 0.077 0.184 XR28L10 92Y61 Prob 0.08040.0512 0.0009 0.1706 XR28L10 92Y70 Mean1 35.87 17.56 6.26 4.66 XR28L1092Y70 Mean2 33.22 17.85 10.14 4.51 XR28L10 92Y70 #Locs 10 10 8 8 XR28L1092Y70 #Reps 11 11 10 10 XR28L10 92Y70 #Years 1 1 1 1 XR28L10 92Y70 %Wins100 0 100 37.5 XR28L10 92Y70 Diff 2.65 −0.29 3.88 −0.14 XR28L10 92Y70 SEDiff 0.131 0.054 0.232 0.153 XR28L10 92Y70 Prob 0 0.0004 0 0.3781XR28L10 92Y80 Mean1 35.87 17.56 6.19 4.55 XR28L10 92Y80 Mean2 35.2717.99 10.76 4.81 XR28L10 92Y80 #Locs 10 10 10 10 XR28L10 92Y80 #Reps 1111 12 12 XR28L10 92Y80 #Years 1 1 2 2 XR28L10 92Y80 %Wins 90 20 100 60XR28L10 92Y80 Diff 0.6 −0.43 4.56 0.26 XR28L10 92Y80 SE Diff 0.198 0.1180.23 0.15 XR28L10 92Y80 Prob 0.0143 0.0051 0 0.1115 R181 R182 R183 pctpct pct Variety1 Variety2 Statistic ABS ABS ABS XR28L10 92M82 Mean179.74 6.69 2.48 XR28L10 92M82 Mean2 24.91 57.78 2.82 XR28L10 92M82 #Locs8 8 8 XR28L10 92M82 #Reps 10 10 10 XR28L10 92M82 #Years 1 1 1 XR28L1092M82 %Wins 0 100 100 XR28L10 92M82 Diff −54.83 51.09 0.34 XR28L10 92M82SE Diff 0.92 0.801 0.039 XR28L10 92M82 Prob 0 0 0.0001 XR28L10 92M91Mean1 79.74 6.69 2.48 XR28L10 92M91 Mean2 22.76 54.33 7.73 XR28L10 92M91#Locs 8 8 8 XR28L10 92M91 #Reps 10 10 10 XR28L10 92M91 #Years 1 1 1XR28L10 92M91 %Wins 0 100 100 XR28L10 92M91 Diff −56.97 47.64 5.24XR28L10 92M91 SE Diff 1.139 1.092 0.116 XR28L10 92M91 Prob 0 0 0 XR28L1092Y61 Mean1 79.74 6.69 2.48 XR28L10 92Y61 Mean2 79.36 6.89 2.53 XR28L1092Y61 #Locs 8 8 8 XR28L10 92Y61 #Reps 10 10 10 XR28L10 92Y61 #Years 1 11 XR28L10 92Y61 %Wins 62.5 50 87.5 XR28L10 92Y61 Diff −0.38 0.2 0.04XR28L10 92Y61 SE Diff 0.749 0.592 0.037 XR28L10 92Y61 Prob 0.6262 0.74560.2771 XR28L10 92Y70 Mean1 79.74 6.69 2.48 XR28L10 92Y70 Mean2 22.5754.24 8.45 XR28L10 92Y70 #Locs 8 8 8 XR28L10 92Y70 #Reps 10 10 10XR28L10 92Y70 #Years 1 1 1 XR28L10 92Y70 %Wins 0 100 100 XR28L10 92Y70Diff −57.17 47.55 5.97 XR28L10 92Y70 SE Diff 1.071 0.858 0.087 XR28L1092Y70 Prob 0 0 0 XR28L10 92Y80 Mean1 80.45 6.23 2.42 XR28L10 92Y80 Mean223.29 52.67 8.38 XR28L10 92Y80 #Locs 10 10 10 XR28L10 92Y80 #Reps 12 1212 XR28L10 92Y80 #Years 2 2 2 XR28L10 92Y80 %Wins 0 100 100 XR28L1092Y80 Diff −57.17 46.44 5.95 XR28L10 92Y80 SE Diff 0.989 0.813 0.067XR28L10 92Y80 Prob 0 0 0

TABLE 3 Soybean SSR Marker Set SAC1006 SATT129 SATT243 SATT334 SAC1611SATT130 SATT247 SATT335 SAC1634 SATT131 SATT249 SATT336 SAC1677 SATT133SATT250 SATT338 SAC1699 SATT142 SATT251 SATT339 SAC1701 SATT144 SATT255SATT343 SAC1724 SATT146 SATT256 SATT346 SAT_084 SATT147 SATT257 SATT347SAT_090 SATT150 SATT258 SATT348 SAT_104 SATT151 SATT259 SATT352 SAT_117SATT153 SATT262 SATT353 SAT_142-DB SATT155 SATT263 SATT355 SAT_189SATT156 SATT264 SATT356 SAT_222-DB SATT165 SATT265 SATT357 SAT_261SATT166 SATT266 SATT358 SAT_270 SATT168 SATT267 SATT359 SAT_271-DBSATT172 SATT270 SATT361 SAT_273-DB SATT175 SATT272 SATT364 SAT_275-DBSATT181 SATT274 SATT367 SAT_299 SATT183 SATT279 SATT369 SAT_301 SATT186SATT280 SATT373 SAT_311-DB SATT190 SATT282 SATT378 SAT_317 SATT191SATT284 SATT380 SAT_319-DB SATT193 SATT285 SATT383 SAT_330-DB SATT195SATT287 SATT385 SAT_331-DB SATT196 SATT292 SATT387 SAT_343 SATT197SATT295 SATT389 SAT_351 SATT199 SATT299 SATT390 SAT_366 SATT202 SATT300SATT391 SAT_381 SATT203 SATT307 SATT393 SATT040 SATT204 SATT314 SATT398SATT042 SATT212 SATT319 SATT399 SATT050 SATT213 SATT321 SATT406 SATT092SATT216 SATT322 SATT409 SATT102 SATT219 SATT326 SATT411 SATT108 SATT221SATT327 SATT412 SATT109 SATT225 SATT328 SATT413 SATT111 SATT227 SATT330SATT414 SATT115 SATT228 SATT331 SATT415 SATT122 SATT230 SATT332 SATT417SATT127 SATT233 SATT333 SATT418 SATT420 SATT508 SATT583 SATT701 SATT421SATT509 SATT584 SATT708-TB SATT422 SATT510 SATT586 SATT712 SATT423SATT511 SATT587 SATT234 SATT429 SATT512 SATT590 SATT240 SATT431 SATT513SATT591 SATT242 SATT432 SATT514 SATT594 SATT433 SATT515 SATT595 SATT436SATT517 SATT596 SATT440 SATT519 SATT597 SATT441 SATT522 SATT598 SATT442SATT523 SATT601 SATT444 SATT524 SATT602 SATT448 SATT526 SATT608 SATT451SATT529 SATT613 SATT452 SATT533 SATT614 SATT454 SATT534 SATT617 SATT455SATT536 SATT618 SATT457 SATT537 SATT628 SATT460 SATT540 SATT629 SATT461SATT544 SATT630 SATT464 SATT545 SATT631 SATT466 SATT546 SATT632-TBSATT467 SATT548 SATT633 SATT469 SATT549 SATT634 SATT470 SATT550 SATT636SATT471 SATT551 SATT640-TB SATT473 SATT552 SATT651 SATT475 SATT555SATT654 SATT476 SATT556 SATT655-TB SATT477 SATT557 SATT656 SATT478SATT558 SATT660 SATT479 SATT565 SATT661-TB SATT480 SATT566 SATT662SATT487 SATT567 SATT665 SATT488 SATT568 SATT666 SATT491 SATT569 SATT667SATT492 SATT570 SATT672 SATT493 SATT572 SATT675 SATT495 SATT573 SATT677SATT497 SATT576 SATT678 SATT503 SATT578 SATT680 SATT506 SATT581 SATT684SATT507 SATT582 SATT685

Breeding History

Variety XR28L10 evolved from a cross of 93M13 x SP11513488 as shown inTable 4.

TABLE 4 Phase Methodology crossing Bi-parental cross F1 Grow out ofindividual F1 plants to create F2 seed F2 Modified single seed descentF3 Modified single seed descent F4 Single plant selection for progenyrow yield test F4:F5 Progeny row yield test R1 Purification Single plantpurification R1 Yield Test Retest at multiple locations R2 PurificationPlant Row Purification R2.5 Increase 1.0 acre Bulk purification increaseR3 Yield Test Wide area testing R4 Yield Test Wide area testing

All publications, patents and patent applications mentioned in thespecification are indicative of the level of those skilled in the art towhich this invention pertains. All such publications, patents and patentapplications are incorporated by reference herein for the purpose citedto the same extent as if each was specifically and individuallyindicated to be incorporated by reference herein.

The foregoing invention has been described in detail by way ofillustration and example for purposes of clarity and understanding. Asis readily apparent to one skilled in the art, the foregoing are onlysome of the methods and compositions that illustrate the embodiments ofthe foregoing invention. It will be apparent to those of ordinary skillin the art that variations, changes, modifications and alterations maybe applied to the compositions and/or methods described herein withoutdeparting from the true spirit, concept and scope of the invention.

1. Soybean variety XR28L10, representative seed of said soybean varietyXR28L10 having been deposited under ATCC Accession Number PTA-12685. 2.A seed of the soybean variety of claim
 1. 3. The seed of claim 2,further comprising a transgene.
 4. The seed of claim 3, wherein thetransgene confers a trait selected from the group consisting of malesterility, site-specific recombination, abiotic stress tolerance,altered phosphorus, altered antioxidants, altered fatty acids, alteredessential amino acids, altered carbohydrates, herbicide resistance,insect resistance and disease resistance.
 5. A soybean plant, or a partthereof, produced by growing the seed of claim
 2. 6. A tissue cultureproduced from the soybean variety of claim
 1. 7. A method for developinga second soybean plant comprising applying plant breeding techniques toa first soybean plant, or parts thereof, wherein said first soybeanplant is the soybean plant of claim 5, and wherein application of saidtechniques results in development of said second soybean plant.
 8. Amethod for producing soybean seed comprising crossing two soybean plantsand harvesting the resultant soybean seed, wherein at least one soybeanplant is the soybean plant of claim
 5. 9. The soybean seed produced bythe method of claim
 8. 10. A soybean plant, or a part thereof, producedby growing said seed of claim
 9. 11. A method for developing a secondsoybean plant in a soybean plant breeding program comprising applyingplant breeding techniques to a first soybean plant, or parts thereof,wherein said first soybean plant is the soybean plant of claim 10, andwherein application of said techniques results in development of saidsecond soybean plant.
 12. A method of producing a soybean plantcomprising a locus conversion, the method comprising introducing a locusconversion into the plant of claim 5, wherein said locus conversionprovides a trait selected from the group consisting of male sterility,site-specific recombination, abiotic stress tolerance, alteredphosphorus, altered antioxidants, altered fatty acids, altered essentialamino acids, altered carbohydrates, herbicide resistance, insectresistance, and disease resistance.
 13. A herbicide resistant soybeanplant produced by the method of claim
 12. 14. A disease resistantsoybean plant produced by the method of claim
 12. 15. An insectresistant soybean plant produced by the method of claim
 12. 16. Thesoybean plant of claim 15, wherein the locus conversion comprises atransgene encoding a Bacillus thuringiensis (Bt) endotoxin.
 17. Theplant of claim 5, further comprising a transgene.
 18. The plant of claim17, wherein the transgene confers a trait selected from the groupconsisting of male sterility, site-specific recombination, abioticstress tolerance, altered phosphorus, altered antioxidants, alteredfatty acids, altered essential amino acids, altered carbohydrates,herbicide resistance, insect resistance, and disease resistance.
 19. Amethod for developing a second soybean plant comprising applying plantbreeding techniques to a first soybean plant, or parts thereof, whereinsaid first soybean plant is the soybean plant of claim 17, and whereinapplication of said techniques results in development of said secondsoybean plant.
 20. A soybean plant, or a part thereof, expressing allthe physiological and morphological characteristics of soybean varietyXR28L10, representative seed of said soybean variety XR28L10 having beendeposited under ATCC Accession Number PTA-12685.